Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 169, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/62245
Keywords
-
Categories
Funding
- Labex Ecofect of Universite de Lyon within the program Investissements d'Avenir [ANR-11-LABX-0048, ANR-11-IDEX-0007]
- Agence Nationale des Recherches sur le SIDA et les Hepatites Virales [ANRS-ECTZ3306]
- European Research Council under European Union's Horizon 2020 research and innovation programs [ERC-StG-LS6-805500]
- Fondation FINOVI
Ask authors/readers for more resources
The CRISPR-Cas system has brought democratization of genome editing in eukaryotic cells, but the delivery of Cas9 protein and sgRNA into target cells remains a technical challenge. Nanoblades, based on MLV, have been developed to overcome issues with traditional viral vectors and can efficiently deliver Cas9 protein and guide RNA without the need for a coding transgene.
The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas system has democratized genome-editing in eukaryotic cells and led to the development of numerous innovative applications. However, delivery of the Cas9 protein and single-guide RNA (sgRNA) into target cells can be technically challenge. Classical viral vectors, such as those derived from lentiviruses (LVs) or adeno-associated viruses (AAVs), allow for efficient delivery of transgenes coding for the Cas9 protein and its associated sgRNA in many primary cells and in vivo. Nevertheless, these vectors can suffer from drawbacks such as integration of the transgene in the target cell genome, a limited cargo capacity, and long-term expression of the Cas9 protein and guide RNA in target cells. To overcome some of these problems, a delivery vector based on the murine Leukemia virus (MLV) was developed to package the Cas9 protein and its associated guide RNA in the absence of any coding transgene. By fusing the Cas9 protein to the C-terminus of the structural protein Gag from MLV, virus-like particles (VLPs) loaded with the Cas9 protein and sgRNA (named Nanoblades) were formed. Nanoblades can be collected from the culture medium of producer cells, purified, quantified, and used to transduce target cells and deliver the active Cas9/sgRNA complex. Nanoblades deliver their ribonucleoprotein (RNP) cargo transiently and rapidly in a wide range of primary and immortalized cells and can be programmed for other applications, such as transient transcriptional activation of targeted genes, using modified Cas9 proteins. Nanoblades are capable of in vivo genome-editing in the liver of injected adult mice and in oocytes to generate transgenic animals. Finally, they can be complexed with donor DNA for transfection-free homology-directed repair. Nanoblade preparation is simple, relatively low-cost, and can be easily carried out in any cell biology laboratory.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available