Journal
GENES
Volume 12, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/genes12040568
Keywords
brown rot; stone fruit; annotation; necrosis; cell-death
Categories
Funding
- European Union [741964]
- Marie Curie Actions (MSCA) [741964] Funding Source: Marie Curie Actions (MSCA)
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This study aimed to generate a high-quality genome of M. fructicola and to identify genes that may contribute to pathogen virulence. Using PacBio sequencing technology, the genome of M. fructicola was assembled, resulting in 10,086 trustworthy gene models. A set of 134 putative effectors was defined, with some triggering necrotic lesions when transiently expressed in Nicotiana benthamiana plants. This research will help better understand the interaction between M. fructicola and stone fruit host plants.
Brown rot is the most economically important fungal disease of stone fruits and is primarily caused by Monilinia laxa and Monlinia fructicola. Both species co-occur in European orchards although M. fructicola is considered to cause the most severe yield losses in stone fruit. This study aimed to generate a high-quality genome of M. fructicola and to exploit it to identify genes that may contribute to pathogen virulence. PacBio sequencing technology was used to assemble the genome of M. fructicola. Manual structural curation of gene models, supported by RNA-Seq, and functional annotation of the proteome yielded 10,086 trustworthy gene models. The genome was examined for the presence of genes that encode secreted proteins and more specifically effector proteins. A set of 134 putative effectors was defined. Several effector genes were cloned into Agrobacterium tumefaciens for transient expression in Nicotiana benthamiana plants, and some of them triggered necrotic lesions. Studying effectors and their biological properties will help to better understand the interaction between M. fructicola and its stone fruit host plants.
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