Journal
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
Volume 28, Issue 5, Pages 2739-2746Publisher
ELSEVIER
DOI: 10.1016/j.sjbs.2021.03.043
Keywords
Skin cancer; Phytoestrogens; Coumestrol; Mitochondrial apoptosis; Cell cycle
Categories
Ask authors/readers for more resources
The study found that the phytoestrogen coumestrol has significant toxicity against human skin cancer cells, inducing cell cytotoxicity through apoptosis stimulation. Coumestrol also inhibits cell migration and invasion in skin cancer cells while blocking the m-TOR/PI3K/AKT signaling pathway.
The current study was undertaken to investigate anticancer activity of coumestrol phytoestrogen against human skin cancer. MTT assay was performed for cell viability assessment and clonogenic assay for cell colony formation assessment. Apoptosis was analysed by Annexin V/FITC staining, AO/EB staining and western blotting assays. Effects on the m-TOR/PI3K/AKT signalling pathway were investigated by western blotting. Results indicated that coumestrol induced significant toxicity in human skin cancer cells in contrast to mouse skin cancer cells. The proliferation rate in normal skin cells remained almost intact. Annexin V-FITC and AO/EB staining assays indicated coumestrol induced cytotoxicity in skin cancer cells is mediated through apoptosis stimulation. The apoptosis in skin cancer cells was mediated through caspase-activation. Cell migration and invasion was inhibited by coumestrol in human skin cancer cells via inhibition of MMP-2 and MMP-9 expressions. Moreover, m-TOR/PI3K/AKT signalling pathway in SKEM-5 cells was blocked by coumestrol. (c) 2021 Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available