Journal
FRONTIERS IN PLANT SCIENCE
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2021.664085
Keywords
chloroplast development; genetic mapping; genomic re-sequencing; comparative analysis; luteostrians; albostrians; HvClpC1; Hordeum vulgare
Categories
Funding
- China Agriculture Research System [CARS-05]
- Agricultural Science and Technology Innovation Program of CAAS
- Deutsche Forschungsgemeinschaft grant (DFG) [1102/13-1]
Ask authors/readers for more resources
Utilizing a combination of low-resolution genetic mapping, whole-genome resequencing and comparative functional analyses can help identify candidate genes involved in plastid biology and photosynthesis in crop plants like barley. This approach provides a promising path towards candidate gene identification for mutant genes.
Implementation of next-generation sequencing in forward genetic screens greatly accelerated gene discovery in species with larger genomes, including many crop plants. In barley, extensive mutant collections are available, however, the causative mutations for many of the genes remains largely unknown. Here we demonstrate how a combination of low-resolution genetic mapping, whole-genome resequencing and comparative functional analyses provides a promising path toward candidate identification of genes involved in plastid biology and/or photosynthesis, even if genes are located in recombination poor regions of the genome. As a proof of concept, we simulated the prediction of a candidate gene for the recently cloned variegation mutant albostrians (HvAST/HvCMF7) and adopted the approach for suggesting HvClpC1 as candidate gene for the yellow-green variegation mutant luteostrians.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available