Journal
POLYMERS
Volume 13, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/polym13071026
Keywords
polymeric coating; microarrays; surface chemistry; label-free sensors; binding kinetics; single-particle imaging
Categories
Funding
- National Science Foundation (NSF iCorps Award) [2027109]
- European Union [766466]
- National Science Foundation (NSF-TT PFI Award) [1941195]
- Directorate For Engineering
- Div Of Industrial Innovation & Partnersh [1941195] Funding Source: National Science Foundation
- Dir for Tech, Innovation, & Partnerships
- Translational Impacts [2027109] Funding Source: National Science Foundation
Ask authors/readers for more resources
This article discusses the importance of microarrays in diagnostics and medicine, as well as the latest advances in surface functionalization methods. The focus is on label-free microarrays, examining the impact of surface chemistry on two types of microarray sensors.
The importance of microarrays in diagnostics and medicine has drastically increased in the last few years. Nevertheless, the efficiency of a microarray-based assay intrinsically depends on the density and functionality of the biorecognition elements immobilized onto each sensor spot. Recently, researchers have put effort into developing new functionalization strategies and technologies which provide efficient immobilization and stability of any sort of molecule. Here, we present an overview of the most widely used methods of surface functionalization of microarray substrates, as well as the most recent advances in the field, and compare their performance in terms of optimal immobilization of the bioreceptor molecules. We focus on label-free microarrays and, in particular, we aim to describe the impact of surface chemistry on two types of microarray-based sensors: microarrays for single particle imaging and for label-free measurements of binding kinetics. Both protein and DNA microarrays are taken into consideration, and the effect of different polymeric coatings on the molecules' functionalities is critically analyzed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available