Journal
GENOME MEDICINE
Volume 13, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s13073-021-00862-6
Keywords
Breast cancer; Metastasis; Next generation sequencing; Targetable genes; Prognosis; de novo metastases
Categories
Funding
- 2009 Programme Hospitalier de Recherche Clinique [02-067]
- Agence Nationale de la Recherche (Investissements d'Avenir program) [ANR-10-EQPX-03, ANR-10-INBS-09-08]
- Canceropole Ile-de-France
- SiRIC-Curie program - SiRIC Grant [INCa-DGOS-4654]
- Breast Cancer Research Foundation (BCRF, USA) [BCRF-19-097]
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Prognosis evaluation and therapeutic strategy for advanced breast cancer are mainly based on clinical features and molecular profiling of the primary tumor. A multicenter, prospective clinical trial was conducted to evaluate the impact of metastatic subtyping during the initial metastatic event. The study revealed the heterogeneity of luminal A tumors and late metastases, providing valuable information for prognosis and therapeutic intervention.
BackgroundPrognosis evaluation of advanced breast cancer and therapeutic strategy are mostly based on clinical features of advanced disease and molecular profiling of the primary tumor. Very few studies have evaluated the impact of metastatic subtyping during the initial metastatic event in a prospective study. The genomic landscape of metastatic breast cancer has mostly been described in very advanced, pretreated disease, limiting the findings transferability to clinical use.MethodsWe developed a multicenter, single-arm, prospective clinical trial in order to address these issues. Between November 2010 and September 2013, 123 eligible patients were included. Patients at the first, untreated metastatic event were eligible. All matched primary tumors and metastatic samples were centrally reviewed for pathological typing. Targeted and whole-exome sequencing was applied to matched pairs of frozen tissue. A multivariate overall survival analysis was performed (median follow-up 64months).ResultsPer central review in 84 patients (out of 130), we show that luminal A breast tumors are more prone to subtype switching. By combining targeted sequencing of a 91 gene panel (n=67) and whole-exome sequencing (n=30), a slight excess of mutations is observed in the metastases. Luminal A breast cancer has the most heterogeneous mutational profile and the highest number of mutational signatures, when comparing primary tumor and the matched metastatic tissue. Tumors with a subtype change have more mutations that are private. The metastasis-specific mutation load is significantly higher in late than in de novo metastases. The most frequently mutated genes were TP53 and PIK3CA. The most frequent metastasis-specific druggable genes were PIK3CA, PTEN, KDR, ALK, CDKN2A, NOTCH4, POLE, SETD2, SF3B1, and TSC2. Long-term outcome is driven by a combination of tumor load and metastasis biology.ConclusionsProfiling of the first, untreated, metastatic event of breast cancer reveals a profound heterogeneity mostly in luminal A tumors and in late metastases. Based on this profiling, we can derive information relevant to prognosis and therapeutic intervention, which support current guidelines recommending a biopsy at the first metastatic relapse.Trial registrationThe trial was registered at ClinicalTrials.gov (NCT01956552).
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