KDM6B-dependent chromatin remodeling underpins effective virus-specific CD8+ T cell differentiation

Naive CD8(+) T cell activation results in an autonomous program of cellular proliferation and differentiation. However, the mechanisms that underpin this process are unclear. Here, we profile genome-wide changes in chromatin accessibility, gene transcription, and the deposition of a key chromatin modification (H3K27me3) early after naive CD8(+) T cell activation. Rapid upregulation of the histone demethylase KDM6B prior to the first cell division is required for initiating H3K27me3 removal at genes essential for subsequent T cell differentiation and proliferation. Inhibition of KDM6B-dependent H3K27me3 demethylation limits the magnitude of an effective primary virus-specific CD8(+) T cell response and the formation of memory CD8(+) T cell populations. Accordingly, we define the early spatiotemporal events underpinning early lineage-specific chromatin reprogramming that are necessary for autonomous CD8(+) T cell proliferation and differentiation.

Automated summary

New research has revealed that rapid upregulation of the histone demethylase KDM6B is crucial for initiating H3K27me3 removal and promoting T cell differentiation and proliferation after CD8(+) T cell activation. Inhibiting KDM6B-dependent H3K27me3 demethylation limits the effectiveness of primary virus-specific CD8(+) T cell response and the formation of memory CD8(+) T cell populations. The study defines early spatial and temporal events necessary for chromatin reprogramming in CD8(+) T cells for autonomous proliferation and differentiation.