Journal
ADVANCED HEALTHCARE MATERIALS
Volume 10, Issue 11, Pages -Publisher
WILEY
DOI: 10.1002/adhm.202100034
Keywords
electrochemical biosensors; nucleic acid amplification tests (NAATs); recombinase polymerase amplification (RPA); self‐ assembled monolayers (SAMs); textile microfluidics
Funding
- European Research Council [715268]
- Knut and Alice Wallenberg Foundation
- European Research Council (ERC) [715268] Funding Source: European Research Council (ERC)
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Fiber-based biosensors with microfluidic structures and electroanalytical readout are used for Nucleic Acid Amplification Testing. Two types of fiber-based electrodes are characterized, and a woven electrochemical DNA sensor is fabricated for specific detection of genomic DNA of Staphylococcus epidermidis.
Fiber-based biosensors enable a new approach in analytical diagnostic devices. The majority of textile-based biosensors, however, rely on colorimetric detection. Here a woven biosensor that integrates microfluidics structures in combination with an electroanalytical readout based on a thiol-self-assembled monolayer (SAM) for Nucleic Acid Amplification Testing, NAATs is shown. Two types of fiber-based electrodes are systematically characterized: pure gold microwires (bond wire) and off-the-shelf plasma gold-coated polyester multifilament threads to evaluate their potential to form SAMs on their surface and their electrochemical performance in woven textile. A woven electrochemical DNA (E-DNA) sensor using a SAM-based stem-loop probe-modified gold microwire is fabricated. These sensors can specifically detect unpurified, isothermally amplified genomic DNA of Staphylococcus epidermidis (10 copies/mu L) by recombinase polymerase amplification (RPA). This work demonstrates that textile-based biosensors have the potential for integrating and being employed as automated, sample-to-answer analytical devices for point-of-care (POC) diagnostics.
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