Journal
ACS SYNTHETIC BIOLOGY
Volume 10, Issue 5, Pages 957-963Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.1c00016
Keywords
nootkatone; synthetic biology; Artemisia annua
Categories
Funding
- NSFC [81973420, 81803660]
- National Key Research and Development Project [2019YFE0108700]
- Experimental Technology Research Project of Southwest University [SYJ2020004]
- Natural Science Foundation of Chongqing [cstc2018jcyjAX0328]
- Science Funding of Sichuan Province [2020YJ0171]
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In this study, nootkatone was successfully synthesized in Artemisia annua using synthetic biology methods, with significantly higher yield in engineered plastids compared to cytosol. This research provides a green approach for producing nootkatone in Artemisia annua with great market potential.
Nootkatone is a valuable sesquiterpene widely used in the food, fragrance, and flavor industries. Its price is very high due to its limited production in grapefruit peels or Alaska cypress heartwoods. Chemical synthesis of nootkatone uses heavy metals, highly flammable compounds, and strong oxidants, which cause severe damage to the environment. In this study, nootkatone is synthesized in Artemisia annua, using synthetic biology methods. Engineered Artemisia annua coexpressing valencene synthase (VS) and valencene oxidase (VO) in the cytosol produced nootkatone ranging from 0.89 to 8.52 mu g/g fresh weight (FW). Furthermore, transgenic Artemisia annua coexpressing farnesyl diphosphate synthase (FPS), VS, and VO in plastids produced nootkatone ranging from 12.11 to 47.80 mu g/g FW. These results indicated that engineering nootkatone biosynthesis in plastids was superior to that in the cytosol. Meanwhile, artemisinin production was unaltered in nootkatone-producing Artemisia annua. Our study developed a green approach for producing nootkatone in Artemisia annua with great market potential.
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