Journal
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
Volume 32, Issue 2, Pages 325-333Publisher
SPRINGER HEIDELBERG
DOI: 10.1590/0104-6632.20150322s00003268
Keywords
Trichoderma harzianum; Glycosyl hydrolases; Ethanol precipitation
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Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [CNPq 482177/2011-0]
- FAPESP [2010/08089-2]
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This study aimed to concentrate glycosyl hydrolases produced by Trichoderma harzianum P49P11 by ethanol precipitation. The variables tested besides ethanol concentration were temperature and pH. The precipitation with 90% (v/v) ethanol at pH 5.0 recovered more than 98% of the xylanase activity, regardless of the temperature (5.0, 15.0, or 25.0 degrees C). The maximum recovery of cellulase activity as FPase was 77% by precipitation carried out at this same pH and ethanol concentration but at 5.0 degrees C. Therefore, ethanol precipitation can be considered to be an efficient technique for xylanase concentration and, to a certain extent, also for the cellulase complex.
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