Investigation of the antimicrobial activity and hematological pattern of nano-chitosan and its nano-copper composite

Novel synthesized Chitosan-Copper oxide nanocomposite (Cs-CuO) was prepared using pomegranate peels extract as green precipitating agents to improve the biological activity of Cs-NP's, which was synthesized through the ionic gelation method. The characterization of biogenic nanoparticles Cs-NP's and Cs-CuO-NP's was investigated structurally, morphologically to determine all the significant characters of those nanoparticles. Antimicrobial activity was tested for both Cs-NP's and Cs-CuO-NP's via minimum inhibition concentration and zone analysis against fungus, gram-positive and gram-negative. The antimicrobial test results showed high sensitivity of Cs-CuO-NP's to all microorganisms tested in a concentration less than 20,000 mg/L, while the sensitivity of Cs-NP's against all microorganisms under the test started from a concentration of 20,000-40,000 mg/L except for the C. albicans species. The hematological activity was also tested via measuring the RBCs, platelet count, and clotting time against healthy, diabetic, and hypercholesteremia blood samples. The measurement showed a decrease in RBCs and platelet count by adding Cs-NP's or Cs-CuO-NP's to the three blood samples. Cs-NP's success in decreasing the clotting time for healthy and diabetic blood acting as a procoagulant agent while adding biogenic CuO-NP's to Cs-NP's increased clotting time considering as an anti-coagulant agent for hypercholesteremia blood samples.

Automated summary

The study focused on the synthesis of Chitosan-Copper oxide nanocomposite (Cs-CuO) using pomegranate peels extract as a green precipitating agent to enhance the biological activity of Cs-NP's. The antimicrobial activity of Cs-NP's and Cs-CuO-NP's was tested against various microorganisms, revealing higher sensitivity of Cs-CuO-NP's at lower concentrations. Additionally, hematological activity was assessed by measuring blood parameters in healthy, diabetic, and hypercholesterolemic samples, showing different effects of Cs-NP's and Cs-CuO-NP's on clotting time.