4.7 Article

Intestinal mucosa-derived DNA methylation signatures in the penetrating intestinal mucosal lesions of Crohn's disease

Journal

SCIENTIFIC REPORTS
Volume 11, Issue 1, Pages -

Publisher

NATURE PORTFOLIO
DOI: 10.1038/s41598-021-89087-6

Keywords

-

Funding

  1. Innovation Project of Military Medicine [16CXZ007]
  2. Distinguished Scholars Foundation of Jiangsu Province [JCRCB2016006]
  3. National Major Scientific and Technological Special Project for Significant New Drugs Development [2018ZX09J18111-004]

Ask authors/readers for more resources

This study comprehensively evaluated the genome-wide DNA methylation changes in intestinal mucosa tissue of adult patients with Crohn's disease. Abnormal DNA methylation sites were identified and correlated with genes related to apoptosis, IL-8 production, and inflammatory pathways. The degree of abnormal methylation of MUC1 was negatively correlated with the disease activity of Crohn's disease, suggesting a potential role in disease progression.
The purpose of this study was to evaluate genome-wide DNA methylation changes in intestinal mucosa tissue of adult patients with Crohn's disease comprehensively. DNA methylation chip was used to analyze abnormal methylation sites among penetrating and non-penetrating intestinal mucosa tissue of Crohn's disease and normal intestinal mucosa tissue of healthy controls. Methylation abnormalities of different locus were verified by pyrosequencing and quantitative polymerase chain reaction. Differential DNA methylation sites were participated in the positive regulation of apoptosis and the positive regulation of IL-8 production and were enriched in signaling pathways related to inflammatory bowel disease and extracellular matrix receptor interaction signaling pathways. Correlation analysis showed that the methylation abnormalities of HLA-DRB1 (r=- 0.62, P<0.001), MUC1 (r=- 0.45, P=0.01), YPEL5 (r=- 0.55, P=0.001) and CBLB (r=- 0.62, P<0.001) were significantly negatively correlated with their relative expression levels. The degree of methylation abnormality of MUC1 was negatively correlated with the disease activity score of Crohn's disease (r=- 0.50, P=0.01). Apoptosis, interleukin-8 production and abnormal extracellular matrix might be involved in the mechanism of penetrating intestinal mucosal lesions in Crohn's disease. The degree of abnormal methylation of MUC1 was negatively correlated with the disease activity of Crohn's disease.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available