Cell-Mediated Immunoreactivity of Poly(2-isopropenyl-2-oxazoline) as Promising Formulation for Immunomodulation

Poly(2-isopropenyl-2-oxazoline) (PIPOx) represents a functional polymer with high potential for drug delivery, tissue engineering, and immunomodulation. The immunomodulatory efficiency of the PIPOx formulation has been studied in vitro following splenic cells and RAW 264.7 macrophages exposition. The cell-specific immunomodulative effect on production of Th1, Th2, Th17, and Treg signature cytokines has been demonstrated. The impact on the functionality of PIPOx-sensitized RAW 264.7 macrophages was assessed by cell phagocytosis. Time- and concentration-dependent cell internalization and intracellular organelles colocalization of fluorescently labeled PIPOx has been examined. The in vitro results demonstrated the PIPOx bioavailability and the capability of triggering immune cell responses resulting in the induced production of cell-specific signature interleukins, important prerequisite properties for future potential biomedical applications.

Automated summary

Poly(2-isopropenyl-2-oxazoline) (PIPOx) is a functional polymer with high potential for drug delivery, tissue engineering, and immunomodulation. In vitro studies have shown the immunomodulatory efficiency of PIPOx formulation on various immune cell responses, including the production of specific cytokines and cell phagocytosis. The results demonstrate the bioavailability of PIPOx and its capability to induce immune cell responses, highlighting its potential for future biomedical applications.