Journal
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
Volume 15, Issue 3, Pages 404-+Publisher
J INFECTION DEVELOPING COUNTRIES
DOI: 10.3855/jidc.13573
Keywords
K; pneumoniae; XDR; PDR; AMR; NDM; OXA
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This study characterized two carbapenem-resistant K. pneumoniae strains from the same patient, revealing a high number of antimicrobial resistant genes and virulence factor genes which could potentially lead to life-threatening infections.
Introduction: Infections caused by extensively-drug resistant (XDR) and pan-drug resistant (PDR) Klebsiella pneumoniae represent an emerging threat due to the high associated mortality. This study aimed to characterize two carbapenem resistant K. pneumoniae strains from the same patient, the first being PDR (referred to as IMP 1078b) and the second being XDR (referred to as IMP 1078s) isolated from the same patient. Methodology: Antimicrobial susceptibility testing was done for the 2 K. pneumoniae isolates, followed by carbapenem/beta-lactamase inhibitor combination assay, and fitness cost against cefepime and meropenem. Then, whole-genome sequence analysis was performed to decipher the molecular mechanisms behind the high level of resistance recorded in both isolates. Finally, qRT-PCR was done for beta-lactam resistant genes. Results: This is the first report about a K. pneumoniae isolate harboring 47 antimicrobial resistance genes and having type IV pilli (Yersinia) and the fimbrial adherence determinant Stb (Salmonella) as virulence factors. Further analysis on both isolates are discussed within the article. Conclusions: The co-existence of a high number of antimicrobial resistant (AMR) genes and virulence factor genes may lead to a life threatening invasive and untreatable infection.
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