Journal
CONSERVATION GENETICS RESOURCES
Volume 13, Issue 3, Pages 337-339Publisher
SPRINGER
DOI: 10.1007/s12686-021-01205-8
Keywords
Pacific herring; eDNA; Quantitative PCR; Minor groove binding (MGB) probe
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Funding
- National Research Foundation of Korea Grant - Korean Government [NRF-2019R1F1A1062149]
- Polar Academic Program of Korea Polar Research Institute
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An eDNA assay using qPCR technology was developed for the detection of Pacific herring, showing high sensitivity and specificity. The method was validated using field-collected water samples and demonstrated a non-invasive and non-destructive approach for detection.
An eDNA assay for the detection of Pacific herring (Clupea pallasii) in water samples was developed using quantitative PCR (qPCR) technology. Species-specific primers and a minor groove binding (MGB) probe were designed based on the mitochondrial cytochrome b gene sequence. Upon conducting a cross-species test, the target species DNA was not only detected at low concentrations but also with high specificity. The assay was validated using field-collected water samples from aquatic environments known to be inhabited by Pacific herring and tested positive in samples from Pacific herring-inhabited sites. Our in silico, in vitro, and in situ results highlight the sensitivity of our proposed eDNA-based procedure for the detection of C. pallasii DNA, and could provide a non-invasive and non-destructive approach to detect this species and characterize its distribution in the Jinhaeman Bay, Korea.
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