4.6 Article

Deriving high contrast fluorescence microscopy images through low contrast noisy image stacks

Journal

BIOMEDICAL OPTICS EXPRESS
Volume 12, Issue 9, Pages 5529-5543

Publisher

OPTICAL SOC AMER
DOI: 10.1364/BOE.422747

Keywords

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Funding

  1. Norges Forskningsrad [288082, 804233]
  2. H2020 Excellent Science [749666]
  3. Universitetet i TromsO (Publication fund, Strategic fund)

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Contrast in fluorescence microscopy images can be increased by exploiting the fluctuation of emitters in a stack of images, yielding results comparable to structured illumination microscopy in tissue samples.
Contrast in fluorescence microscopy images allows for the differentiation between different structures by their difference in intensities. However, factors such as point-spread function and noise may reduce it, affecting its interpretability. We identified that fluctuation of emitters in a stack of images can be exploited to achieve increased contrast when compared to the average and Richardson-Lucy deconvolution. We tested our methods on four increasingly challenging samples including tissue, in which case results were comparable to the ones obtained by structured illumination microscopy in terms of contrast. (c) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

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