Journal
NATURE COMMUNICATIONS
Volume 12, Issue 1, Pages -Publisher
NATURE RESEARCH
DOI: 10.1038/s41467-021-22705-z
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Funding
- Spanish Ministry of Economy and Competitiveness [SAF2016-77346-R, PID2019-104477RB-I00, BES-2017-079656]
- ERC [638553-TB-ACCELERATE]
- Spanish Government-FEDER Funds [CPII18/00031, PI16/01511]
- Generalitat Valencia Grant [PROMETEO/2020/012]
- Ministry of Science
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Polyclonal infections, where at least two unrelated strains of the same pathogen are detected in an individual, have been linked to worse clinical outcomes in tuberculosis. Analysis of samples from tuberculosis patients in Georgia by Moreno-Molina et al. showed that the magnitude of polyclonal infections may be underestimated when only testing sputum samples.
Polyclonal infections occur when at least two unrelated strains of the same pathogen are detected in an individual. This has been linked to worse clinical outcomes in tuberculosis, as undetected strains with different antibiotic resistance profiles can lead to treatment failure. Here, we examine the amount of polyclonal infections in sputum and surgical resections from patients with tuberculosis in the country of Georgia. For this purpose, we sequence and analyse the genomes of Mycobacterium tuberculosis isolated from the samples, acquired through an observational clinical study (NCT02715271). Access to the lung enhanced the detection of multiple strains (40% of surgery cases) as opposed to just using a sputum sample (0-5% in the general population). We show that polyclonal infections often involve genetically distant strains and can be associated with reversion of the patient's drug susceptibility profile over time. In addition, we find different patterns of genetic diversity within lesions and across patients, including mutational signatures known to be associated with oxidative damage; this suggests that reactive oxygen species may be acting as a selective pressure in the granuloma environment. Our results support the idea that the magnitude of polyclonal infections in high-burden tuberculosis settings is underestimated when only testing sputum samples. Polyclonal infections occur when at least two unrelated strains of the same pathogen are detected in an individual. Here, Moreno-Molina et al. analyse sputum and surgical resections from tuberculosis patients, showing that the magnitude of polyclonal infections can be underestimated when only testing sputum samples.
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