Journal
NATURE COMMUNICATIONS
Volume 12, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41467-021-23252-3
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Funding
- Ministry of Education, Youth and Sports of the Czech Republic [LL1602]
- Czech Science Foundation [18-19705S]
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The study introduces a high-speed photothermal spatial light modulator capable of generating a step-like wavefront change without diffraction artifacts, used for quantitative phase imaging to capture nanometer-scale 3D displacements.
Spatial light modulators have become an essential tool for advanced microscopy, enabling breakthroughs in 3D, phase, and super-resolution imaging. However, continuous spatial-light modulation that is capable of capturing sub-millisecond microscopic motion without diffraction artifacts and polarization dependence is challenging. Here we present a photothermal spatial light modulator (PT-SLM) enabling fast phase imaging for nanoscopic 3D reconstruction. The PT-SLM can generate a step-like wavefront change, free of diffraction artifacts, with a high transmittance and a modulation efficiency independent of light polarization. We achieve a phase-shift > and a response time as short as 70 mu s with a theoretical limit in the sub microsecond range. We used the PT-SLM to perform quantitative phase imaging of sub-diffractional species to decipher the 3D nanoscopic displacement of microtubules and study the trajectory of a diffusive microtubule-associated protein, providing insights into the mechanism of protein navigation through a complex microtubule network. Here, the authors present a high-speed photothermal spatial light modulator which can generate a step-like wavefront change without diffraction artifacts. They use this to perform quantitative phase imaging, capturing sub-millisecond motion with a nanometer resolution in 3D.
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