Journal
EXPERIMENTAL AND THERAPEUTIC MEDICINE
Volume 21, Issue 6, Pages -Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/etm.2021.10078
Keywords
hypoxia-inducible factor-1α microRNA-32-5p; PTEN; nucleus pulposus cells; PI3K; AKT; mTOR signaling pathway
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Funding
- Natural Science Foundation of Hainan Province [819QN350]
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The study identified 994 significant differentially expressed miRNAs in NPCs, with miR-32-5p being upregulated. PTEN was downregulated in NPCs from non-degenerative disc tissues of young patients. Through regulation of the HIF-1 alpha/miR-32-5p/PTEN/PI3K/AKT/mTOR axis, cell proliferation and apoptosis were controlled in NPCs.
Intervertebral disc degeneration and resulting low back pain arises from the programmed apoptosis of nucleus pulposus cells (NPCs). Recent studies show that hypoxia-inducible factor-1 alpha plays a vital role in the etiology and pathogenesis of disc degeneration. However, the underlying mechanism of HIF-1 alpha in NPCs is unclear. The present study identified 994 significant differentially expressed miRNAs by analyzing microarray data downloaded from the Gene Expression Omnibus database. MicroRNA(miR)-32-5p expression was 2.81-fold upregulated in NPCs compared with that of the healthy control tissues (P<0.05). A total of 331 significant differentially expressed mRNAs were identified, and PTEN was downregulated in NPCs of non-degenerative disc tissues from young patients. miR-32-5p was predicted to target the PTEN 3'-untranslated region (UTR). To confirm these results, in-vitro experiments investigating the molecular function of miR-32-5p and PTEN were performed. Furthermore, hypoxia induced miR-32-5p and PTEN expression. HIF-1 alpha inhibited NPC proliferation and promoted cell apoptosis by regulating miR-32-5p and PTEN. miR-32-5p promoted NPC proliferation and decreased cell apoptosis. Next, it was verified whether miR-32-5p targeted the PTEN 3'-UTR using dual-luciferase reporter assays. Finally, it was observed that PI3K/AKT/mTOR signaling pathway was upregulated by a miR-32-5p mimic, which improved cell proliferation and decreased apoptosis. Importantly, PTEN was downregulated in these experiments; and inhibition of miR-32-5p had the opposite effect. Overall, these results demonstrate that HIF-1 alpha regulates cell proliferation and apoptosis by controlling the miR-32-5p/PTEN/PI3K/AKT/mTOR axis in NPCs.
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