4.7 Article

CircSPI1 acts as an oncogene in acute myeloid leukemia through antagonizing SPI1 and interacting with microRNAs

Journal

CELL DEATH & DISEASE
Volume 12, Issue 4, Pages -

Publisher

SPRINGERNATURE
DOI: 10.1038/s41419-021-03566-2

Keywords

-

Categories

Funding

  1. National Natural Science Foundation Grants of China [81890994, 81770153, 81530003, 81911530240]
  2. National Key Research and Development Program [2019YFA0905900]

Ask authors/readers for more resources

This study reveals that circSPI1, a circular RNA derived from the SPI1 gene, is highly expressed in AML and acts as an oncogene, promoting AML development through multiple interactions. CircSPI1 contributes to myeloid differentiation, proliferation, and apoptosis in AML cells by interacting with various factors, showcasing the complexity of SPI1 gene regulation involving circSPI1.
PU.1 (encoded by SPI1) is essential for myeloid development, and inhibition of its expression and activity can lead to acute myeloid leukemia (AML). The precise regulation of PU.1 expression is crucial for the development of AML, and the discovery of circular RNAs (circRNAs) can add a new layer of information on regulation. Here, we found that circSPI1, the circular RNA derived from the SPI1 gene, is highly expressed in AML but not in normal counterparts. Unlike SPI1, a tumor suppressor and being lowly expressed in AML, we demonstrate that circSPI1 acts as an oncogene, evidenced by the observation that circSPI1 knockdown induces myeloid differentiation and apoptosis of AML cells. We provide mechanistic evidence for multiple regulatory roles of circSPI1 in AML progression. On one hand, circSPI1 contributes to myeloid differentiation of AML cells by interacting with the translation initiation factor eIF4AIII to antagonize PU.1 expression at the translation level. On the other hand, circSPI1 contributes to proliferation and apoptosis by interacting with miR-1307-3p, miR-382-5p, and miR-767-5p; this role is uncoupled with SPI1. Finally, we illustrate the clinical significance of circSPI1 by showing that circSPI1-regulated genes are associated with the clinical outcome of AML patients. Our data provide new insight into the complex SPI1 gene regulation now involving circSPI1.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available