Journal
VIRUSES-BASEL
Volume 13, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/v13050770
Keywords
Hepatitis B virus; capsid assembly modulators; virtual screening; protein-protein interaction; pharmacophore modelling
Categories
Funding
- National Institute of Allergy and Infectious Diseases (NIAID) at the National Institutes of Health (NIH) [R01AI121315]
- Nahmias-Schinazi Distinguished Chair in Research
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Novel HBV CpAM hits were identified using structure-based virtual screening and pharmacophore-guided compound design, showing potent antiviral activity and high stability in ADME assays.
Hepatitis B virus (HBV) capsid assembly modulators (CpAMs) have shown promise as potent anti-HBV agents in both preclinical and clinical studies. Herein, we report our efforts in identifying novel CpAM hits via a structure-based virtual screening against a small molecule protein-protein interaction (PPI) library, and pharmacophore-guided compound design and synthesis. Curated compounds were first assessed in a thermal shift assay (TSA), and the TSA hits were further evaluated in an antiviral assay. These efforts led to the discovery of two structurally distinct scaffolds, ZW-1841 and ZW-1847, as novel HBV CpAM hits, both inhibiting HBV in single-digit mu M concentrations without cytotoxicity at 100 mu M. In ADME assays, both hits displayed extraordinary plasma and microsomal stability. Molecular modeling suggests that these hits bind to the Cp dimer interfaces in a mode well aligned with known CpAMs.
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