Journal
VIRUSES-BASEL
Volume 13, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/v13050779
Keywords
gene editing; CRISPR; Cas9; virus; gene function; vaccine
Categories
Funding
- National Natural Science Foundation of China [U1604232]
- Henan Thousand Talents Program-Leading Talents in Basic Research (2019-2020)
- Natural Science Foundation of Henan Province [212300410359]
- Key R&D and Promotion Project of Henan Province [212102110097]
- Fund for Distinguished Young Scholars from Henan Academy of Agricultural Sciences [2019JQ01]
- BBSRC Newton Fund Joint Centre Awards on UK-China Centre of Excellence for Research on Avian Diseases
Ask authors/readers for more resources
CRISPR/Cas9-based gene-editing techniques have been widely applied in biological sciences, particularly in modifying viral genomes, to reveal gene function, virus pathogenesis, and play a crucial role in vaccine development.
In recent years, the CRISPR/Cas9-based gene-editing techniques have been well developed and applied widely in several aspects of research in the biological sciences, in many species, including humans, animals, plants, and even in viruses. Modification of the viral genome is crucial for revealing gene function, virus pathogenesis, gene therapy, genetic engineering, and vaccine development. Herein, we have provided a brief review of the different technologies for the modification of the viral genomes. Particularly, we have focused on the recently developed CRISPR/Cas9-based gene-editing system, detailing its origin, functional principles, and touching on its latest achievements in virology research and applications in vaccine development, especially in large DNA viruses of humans and animals. Future prospects of CRISPR/Cas9-based gene-editing technology in virology research, including the potential shortcomings, are also discussed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available