☆ 4.6 Article

Evaluation of an Automated High-Throughput Liquid-Based RNA Extraction Platform on Pooled Nasopharyngeal or Saliva Specimens for SARS-CoV-2 RT-PCR

VIRUSES-BASEL (2021)

Related references

Note: Only part of the references are listed.

Article Immunology

Unique Clusters of Severe Acute Respiratory Syndrome Coronavirus 2 Causing a Large Coronavirus Disease 2019 Outbreak in Hong Kong

Kelvin Kai-Wang To et al.

Summary: After a period of calm, a significant COVID-19 outbreak occurred in Hong Kong in July 2020 following the easing of social distancing measures. Unique phylogenetic clusters of SARS-CoV-2 were identified among locally acquired cases, with majority of genomes belonging to the HK1 cluster, which is related to SARS-CoV-2 strains reported overseas.

CLINICAL INFECTIOUS DISEASES (2021)

Add to Collection

Article Multidisciplinary Sciences

A pooled testing strategy for identifying SARS-CoV-2 at low prevalence

Leon Mutesa et al.

Summary: To suppress SARS-CoV-2 infections, rapid identification and isolation are needed. Costly RT-PCR tests can be reduced by pooling subsamples, while maintaining sensitivity through parallel searches. This approach requires fewer tests and minimal delays in identifying infected individuals.

NATURE (2021)

Add to Collection

Article Microbiology

Pooled Saliva Specimens for SARS-CoV-2 Testing

Bidisha Barat et al.

Summary: Saliva testing showed high positive and negative agreement compared to nasopharyngeal swabs, especially for samples with moderate to high viral load. Pooling saliva specimens for testing exhibited decreased sensitivity compared to individual testing, but could still detect individuals with higher viral loads in an asymptomatic screening program. This method eliminates the need for swabs or viral transport medium and may improve screening compliance among individuals averse to nasal collections.

JOURNAL OF CLINICAL MICROBIOLOGY (2021)

Add to Collection

Article Biochemical Research Methods

SARS-CoV-2: Comparative analysis of different RNA extraction methods

Cecilia Ambrosi et al.

Summary: This study compared the efficiency of RNA extraction using three different commercial kits and an in-house protocol for SARS-CoV-2 detection. The tested commercial kits can be used with modifications for rRT-PCR approaches, with some differences in RNA yields observed. The EXTRAzol reagent was found to be less efficient due to the phase separation principle.

JOURNAL OF VIROLOGICAL METHODS (2021)

Add to Collection

Review Immunology

Lessons learned 1 year after SARS-CoV-2 emergence leading to COVID-19 pandemic

Kelvin Kai-Wang To et al.

Summary: The lack of modern medical management and vaccines could lead to the COVID-19 pandemic reaching the levels of past deadly outbreaks like the 1894 plague and 1918-A(H1N1) influenza pandemic. The presence of multiple coronaviruses with pandemic potential in animals, coupled with environmental factors like the mixing and selling of wild mammals with poor hygiene practices in urban markets, pose a significant threat to global health. The emergence of new viruses and the challenges of mass testing, contact tracing, treatment, and potential development of vaccine-resistant virus variants highlight the importance of global preparedness in combating infectious diseases.

EMERGING MICROBES & INFECTIONS (2021)

Add to Collection

Article Multidisciplinary Sciences

Increased mortality in community-tested cases of SARS-CoV-2 lineage B.1.1.7

Nicholas G. Davies et al.

Summary: Studies have shown that the SARS-CoV-2 B.1.1.7 lineage is more transmissible and may cause more severe illness compared to pre-existing variants.

NATURE (2021)

Add to Collection

Article Immunology

Performance of Nucleic Acid Amplification Tests for Detection of Severe Acute Respiratory Syndrome Coronavirus 2 in Prospectively Pooled Specimens

Hannah Wang et al.

Summary: Pooled nucleic acid amplification tests have the potential to increase the availability of testing for SARS-CoV-2 at a lower cost, but the impact of dilution on analytical sensitivity needs further characterization. Positive percent agreement (PPA) varied based on the number of pooled samples and factors like cycle threshold distribution and assay limit of detection, highlighting the complex nature of pooled testing strategies. Developing and validating stochastic simulation models can help estimate the effects of dilution on PPA and efficiency of pooled testing algorithms.

EMERGING INFECTIOUS DISEASES (2021)

Add to Collection

Article Immunology

Consistent Detection of 2019 Novel Coronavirus in Saliva

Kelvin Kai-Wang To et al.

CLINICAL INFECTIOUS DISEASES (2020)

Add to Collection

Article Microbiology

Improved Molecular Diagnosis of COVID-19 by the Novel, Highly Sensitive and Specific COVID-19-RdRp/Hel Real-Time Reverse Transcription-PCR Assay Validated In Vitro and with Clinical Specimens

Jasper Fuk-Woo Chan et al.

JOURNAL OF CLINICAL MICROBIOLOGY (2020)

Add to Collection

Article Immunology

Evaluation of COVID-19 RT-qPCR Test in Multi sample Pools

Idan Yelin et al.

CLINICAL INFECTIOUS DISEASES (2020)

Add to Collection

Article Infectious Diseases

An alternative workflow for molecular detection of SARS-CoV-2-escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020

Anna S. Fomsgaard et al.

EUROSURVEILLANCE (2020)

Add to Collection

Article Biochemistry & Molecular Biology

Development of a Novel, Genome Subtraction-Derived, SARS-CoV-2-Specific COVID-19-nsp2 Real-Time RT-PCR Assay and Its Evaluation Using Clinical Specimens

Cyril Chik-Yan Yip et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

Add to Collection

Article Immunology

Evaluating the use of posterior oropharyngeal saliva in a point-of-care assay for the detection of SARS-CoV-2

Jonathan Hon-Kwan Chen et al.

EMERGING MICROBES & INFECTIONS (2020)

Add to Collection

Article Infectious Diseases

Large-scale implementation of pooled RNA extraction and RT-PCR for SARS-CoV-2 detection

R. Ben-Ami et al.

CLINICAL MICROBIOLOGY AND INFECTION (2020)

Add to Collection

Article Biochemistry & Molecular Biology

Development and Evaluation of Novel and Highly Sensitive Single-Tube Nested Real-Time RT-PCR Assays for SARS-CoV-2 Detection

Cyril Chik-Yan Yip et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

Add to Collection

Article Virology

Evaluation of simple nucleic acid extraction methods for the detection of SARS-CoV-2 in nasopharyngeal and saliva specimens during global shortage of extraction kits

Allen Wing-Ho Chu et al.

JOURNAL OF CLINICAL VIROLOGY (2020)

Add to Collection

Article Immunology

Group Testing for Severe Acute Respiratory Syndrome-Coronavirus 2 to Enable Rapid Scale-up of Testing and Real-Time Surveillance of Incidence

Christopher D. Pilcher et al.

JOURNAL OF INFECTIOUS DISEASES (2020)

Add to Collection

Article Virology

Identification of nsp1 gene as the target of SARS-CoV-2 real-time RT-PCR using nanopore whole-genome sequencing

Wan-Mui Chan et al.

JOURNAL OF MEDICAL VIROLOGY (2020)

Add to Collection

Letter Infectious Diseases

Pooling of samples for testing for SARS-CoV-2 in asymptomatic people

Baijayantimala Mishra et al.

LANCET INFECTIOUS DISEASES (2020)

Add to Collection

Letter Medicine, General & Internal

Saliva or Nasopharyngeal Swab Specimens for Detection of SARS-CoV-2

Anne L. Wyllie et al.

NEW ENGLAND JOURNAL OF MEDICINE (2020)

Add to Collection

Editorial Material Critical Care Medicine

Pooled saliva samples for COVID-19 surveillance programme

Andrew Fogarty et al.

LANCET RESPIRATORY MEDICINE (2020)

Add to Collection

Article Multidisciplinary Sciences

Post-lockdown SARS-CoV-2 nucleic acid screening in nearly ten million residents of Wuhan, China

Shiyi Cao et al.

NATURE COMMUNICATIONS (2020)

Add to Collection

Article Public, Environmental & Occupational Health

COVID-19 in New Zealand and the impact of the national response: a descriptive epidemiological study

Sarah Jefferies et al.

LANCET PUBLIC HEALTH (2020)

Add to Collection

Article Dentistry, Oral Surgery & Medicine

Evaluation of intravirion human immunodeficiency virus type 1 RNA degradation activity in saliva by an RNA cleavage quantification method

Masaaki Murayama et al.

ORAL SCIENCE INTERNATIONAL (2020)

Add to Collection

Article Pathology

Analytical Comparison of Methods for Extraction of Short Cell-Free DNA from Urine

Amy Oreskovic et al.

JOURNAL OF MOLECULAR DIAGNOSTICS (2019)

Add to Collection

Article Genetics & Heredity

Direct PCR amplification of DNA from human bloodstains, saliva, and touch samples collected with microFLOQ (R) swabs

Angie Ambers et al.

FORENSIC SCIENCE INTERNATIONAL-GENETICS (2018)

Add to Collection

Article Immunology

Additional molecular testing of saliva specimens improves the detection of respiratory viruses

Kelvin Kw To et al.

EMERGING MICROBES & INFECTIONS (2017)

Add to Collection

Review Microbiology

The Interaction between Respiratory Pathogens and Mucus

Mark Zanin et al.

CELL HOST & MICROBE (2016)

Add to Collection

Article Chemistry, Physical

DNA Adsorption to and Elution from Silica Surfaces: Influence of Amino Acid Buffers

Peter E. Vandeventer et al.

JOURNAL OF PHYSICAL CHEMISTRY B (2013)

Add to Collection

Article Dentistry, Oral Surgery & Medicine

The effect of saliva specimen collection, handling and storage protocols on hepatitis C virus (HCV) RNA detection by PCR

KM Roy et al.

ORAL DISEASES (2010)

Add to Collection

© Peeref 2019-2024. All rights reserved.