Journal
VIRUSES-BASEL
Volume 13, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/v13050900
Keywords
dengue; maternal immunity; mast cells; vaccines
Categories
Funding
- National Medical Research Council of Singapore [CBRG/0065/2014]
- Singapore Ministry of Education [T2EP30120-0029]
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Sub-neutralizing concentrations of antibodies in dengue infected patients are a major risk factor for the development of dengue hemorrhagic fever and dengue shock syndrome. A mouse model lacking mast cells (MCs) and type I and II interferon receptors was used to study the influence of MCs in a maternal antibody-dependent severe dengue model. The results demonstrate complex interactions between MCs and interferon signaling in influencing the role of maternal antibodies in DENV-induced disease severity.
Sub-neutralizing concentrations of antibodies in dengue infected patients is a major risk factor for the development of dengue hemorrhagic fever and dengue shock syndrome. Here, we describe a mouse model with a deficiency in mast cells (MCs) in addition to a deficiency in Type-I and II IFN receptors for studying dengue virus (DENV) infection. We used this model to understand the influence of MCs in a maternal antibody-dependent model of severe dengue, where offspring born to DENV-immune mothers are challenged with a heterologous DENV serotype. Mice lacking both MCs and IFN receptors were found susceptible to primary DENV infection and showed morbidity and mortality. When these mice were immunized, pups born to DENV-immune mothers were found to be protected for a longer duration from a heterologous DENV challenge. In the absence of MCs and type-I interferon signaling, IFN-gamma was found to protect pups born to naive mothers but had the opposite effect on pups born to DENV-immune mothers. Our results highlight the complex interactions between MCs and IFN-signaling in influencing the role of maternal antibodies in DENV-induced disease severity.
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