Journal
ZOOTAXA
Volume 4951, Issue 3, Pages 559-570Publisher
MAGNOLIA PRESS
DOI: 10.11646/zootaxa.4951.3.8
Keywords
Diptera; Chironomidae; Diamesinae; Pagastia; taxonomy; new species; key; DNA barcoding; phylogenetic relationships; Central Asia; ?triops@yandex; ru
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Chironomids of the genus Pagastia Oliver from the mountains of Central Asia were revised using morphological characters and molecular data. New species were described, an updated key was provided, and a phylogenetic framework was reconstructed based on two mitochondrial genes. Phylogenetic trees of some known species were reconstructed using Bayesian inference and Maximum Likelihood methods, showing interspecific distances corresponding to species level.
Chironomids of the genus Pagastia Oliver (Diamesinae, Diamesini) from the mountains of Central Asia are revised using both morphological characters and molecular data. Illustrated descriptions of the adult male Pagastia (P.) caelestomontana sp. nov. from Kirgizstan and Tajikistan, P. (P.) hanseni sp. nov. from Tajikistan, and record of a finding apparently a new species P. (P.) aff. lanceolata (Tokunaga) from Tajikistan as well as an updated a key to the determination of the adult males of all known species of Pagastia are provided. A phylogenetic framework is reconstructed based on two mitochondrial genes cytochrome oxidase subunit I (COI) sequences of 34 samples belonging to 7 species of the genus Pagastia and cytochrome oxidase subunit II (COII) available for most samples. Phylogenetic trees of some known species of the genus Pagastia were reconstructed using the combined dataset and Bayesian inference (BI) and Maximum Likelihood (ML) methods. The interspecific K2P distances between seven Pagastia species including P. (P.) caelestomontana sp. nov., P. (P.) hanseni sp. nov. and undescribed P. (P.) aff. lanceolata (Tokunaga) are 6.3?13.2 which corresponding to species level.
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