Effect of substrate load on anaerobic fermentation of rice straw with rumen liquid as inoculum: Hydrolysis and acidogenesis efficiency, enzymatic activities and rumen bacterial community structure

Rumen liquid is excellent to effectively degrade lignocellulose. In this study, the suitable rice straw load during anaerobic fermentation of rice straw with rumen liquid as inoculum was explored to improve volatile fatty acid (VFA) production. At 10.0% rice straw load, the highest VFA concentration reached 10821.4 mg/L, and acetic acid and propionic acid were the main components. In 10.0% rice straw load system, high concentration of soluble chemical oxygen demand (SCOD) was also observed, and the enzymatic activities at 48 h were higher than those at other rice straw loads. At 10.0% rice straw load, lower diversity and richness of rumen bacteria were found than those at other rice straw loads. Bacteroides, Prevotella, and Ruminococcus were the main rumen bacteria during rice straw degradation, and the rumen bacteria might secret effective lignocellulolytic enzymes to enhance the hydrolysis and acidogenesis of rice straw. The determination of suitable rice straw load will be beneficial to the application of rumen liquid as inoculum in actual production. (c) 2021 Elsevier Ltd. All rights reserved.

Automated summary

This study found that at a 10.0% rice straw load, the highest VFA concentration, mainly composed of acetic acid and propionic acid, was achieved, along with high SCOD concentration and enzyme activities. Lower diversity and richness of rumen bacteria were observed at this load compared to others. Bacteroides, Prevotella, and Ruminococcus were identified as the main rumen bacteria during rice straw degradation.