Journal
VIRUS GENES
Volume 57, Issue 4, Pages 318-326Publisher
SPRINGER
DOI: 10.1007/s11262-021-01842-z
Keywords
MiRNAs; Enterovirus 71; RD cell; VP3; Virus replication
Categories
Funding
- Operating Project of Public Health Emergency Response Mechanism of National Institute for Nutrition and Health, C CDC [131031107000160002]
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In this study, a screening system for miRNAs of target genes was established to investigate the effect of miRNAs on Enterovirus 71 replication in rhabdomyosarcoma cells. The results indicated that miR-18a and miR-452 can repress EV71 virus replication by binding to VP3, suggesting the vital roles of intracellular miRNAs in host-virus interaction.
MicroRNAs (miRNAs) are crucial in the process of host-pathogen interaction. In this study, we established a screening system for miRNAs of target genes to detect the effect of miRNAs on Enterovirus 71 (EV71) replication in rhabdomyosarcoma (RD) cells. A 3'-untranslated region (UTR) dual-luciferase assay was performed to confirm putative miRNA targets in EV71 genome. Firstly, 13 fragments of EV71 genome were inserted into the vector pMIR, and luciferase activities were analyzed to identify the putative miRNAs of target genes. The expression of the reporter protein was significantly downregulated in cells transfected with the vector containing gene VP3. Then we screened for miRNAs that might target to VP3 through online analysis software. In addition, Western blot, real-time PCR, virus titration, and morphological changes were considered to examine the effects of miRNAs on virus replication. The results suggested that miR-18a and miR-452 repress the reproduction of EV71 virus by binding to VP3. Moreover, EV71 infection also affected the expression of endogenous miR-18a and miR-452. In addition, no significant cytotoxic effects were observed. The results from this study suggest that the intracellular miRNAs may play vital roles in the host-virus interaction.
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