Journal
TRANSLATIONAL RESEARCH
Volume 231, Issue -, Pages 55-63Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.trsl.2020.11.008
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Funding
- CNPq scholarship
- Fundacao Araucaria/CAPES (PR, Brazil)
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This study aimed to investigate the role of MBL and ficolin-3 in the complement system activation in COVID-19 patients compared to H1N1 infection and control groups. The findings showed higher tissue expression of proinflammatory cytokines in COVID-19 patients, with particular emphasis on MBL and FCN3. Additionally, certain genetic polymorphisms were associated with high MBL tissue expression in COVID-19 pulmonary samples.
Although some evidence showed the activation of complement systems in COVID19 patients, proinflammatory status and lectin pathway remain unclear. Thus, the present study aimed to demonstrate the role of MBL and ficolin-3 in the complement system activation and compared to pandemic Influenza A virus H1N1 subtype infection (H1N1pdm09) and control patients. A total of 27 lungs formalin-fixed paraffin-embedded samples (10 from H1N1 group, 6 from the COVID-19 group, and 11 from the control group) were analyzed by immunohistochemistry using anti-IL-6, TNF-alfa, CD163, MBL e FCN3 antibodies. Genotyping of target polymorphisms in the MBL2 gene was performed by real-time PCR. Proinflammatory cytokines such as IL 6 and TNF-alpha presented higher tissue expression in the COVID-19 group compared to H1N1 and control groups. The same results were observed for ICAM-1 tissue expression. Increased expression of the FCN3 was observed in the COVID-19 group and H1N1 group compared to the control group. The MBL tissue expression was higher in the COVID-19 group compared to H1N1 and control groups. The genotypes AA for rs180040 (G/A), GG for rs1800451 (G/A) and CC for rs5030737 (T/C) showed a higher prevalence in the COVID-19 group. The intense activation of the lectin pathway, with particular emphasis on the MBL pathway, together with endothelial dysfunction and a massive proinflammatory cytokines production, possibly lead to a worse outcome in patients infected with SARS-Cov-2. Moreover, 3 SNPs of our study presented genotypes that might be correlated with high MBL tissue expression in the COVID-19 pulmonary samples.
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