4.7 Article

Epoxy-functionalized macroporous carbon with embedded platinum nanoparticles for electrochemical detection of telomerase activity via telomerase-triggered catalytic hairpin assembly

Journal

TALANTA
Volume 225, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.talanta.2020.121957

Keywords

Telomerase activity; Electrochemical biosensor; Catalytic hairpin assembly; Ultra-sensitivity detecting

Funding

  1. Priority Strategy Project of Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education [ts2019003]
  2. Natural Science Foundation of Hebei Province [B2019201407, B2018201214]
  3. Science and Technology Project of Hebei Education Department [QN2020153]
  4. Advanced Talents Incubation Program of the Hebei University [801260201026]

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Telomerase-triggered amplification strategy using platinum nanoparticles in an electrochemical biosensor allows for sensitive detection of telomerase activity, providing a sensitive method for biomolecule detection that could be useful for bioanalysis and early clinical diagnoses of diseases.
Telomerase is regarded as a crucial biomarker for the early diagnosis of malignant tumors and a valuable therapeutic target. In this work, a telomerase-triggered amplification strategy was designed on the basis of a catalyzed hairpin assembly (CHA) for bridging a signal probe of platinum nanoparticles (Pt NPs) anchored on three-dimensional (3D) epoxy-functionalized macroporous carbon (Pt/MPC-COOH) in an ultrasensitive electrochemical biosensor. Pt/MPC-COOH nanomaterials with interconnected macroporous structure not only immobilized hairpin DNA probe 2 (H2) via an amide reaction (Pt/MPC-COOH-H2), but they also generated an obvious electrochemical signal in response to acetaminophen (AP) oxidation. After the introduction of telomerase, telomerase primer (TP) was extended to a telomerase extension product (TEP) with several hexamer repeats (TTAGGG)n to initiate the CHA cycle, leading to signal amplification. Subsequently, with the TEP-triggered CHA cycle amplification strategy, a large amount of Pt/MPC-COOH-H2 was introduced on the electrode surface for the construction of the electrochemical platform, which realized the sensitive detection of telomerase activity from 10(2) to10(7) cells mL(-1) with a limit of detection (LOD) of 9.02 cells mL(-1). This strategy provides a sensitive method for the detection of biomolecules that could be useful for bioanalysis and early clinical diagnoses of diseases.

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