Self-organizing map applied to the choice of internal standards for the determination of Cd, Pb, Sn, and platinum group elements by inductively coupled plasma mass spectrometry

The behaviors of internal standards, according to different flow rates of the cell collision gas (He), were studied for the determination of Cd, Pb, Pd, Pt, Rh, and Sn in samples of fish and mollusks by inductively coupled plasma mass spectrometry (ICP-MS). The elements Bi, Ge, In, Sc, and Y were selected as internal standards, considering their masses and first ionization energies. Addition and recovery experiments were carried out at three concentration levels to evaluate the accuracy of the method applied for the analysis of two samples with different matrices. The results were evaluated using a self-organizing map (SOM). The best analyte/IS pairs were as follows: Cd-114(+)/Ge-74(+), Pt-195(+)/Ge-74(+), and Pb-208(+)/Ge-74(+). For Rh-103(+), Pd-106(+), and Sn-120(+), greater accuracy was achieved without use of an internal standard. Helium gas (2.8 mL min(-1)) was used in the collision cell for the analytes, except for Sn, and recoveries ranged from 98 to 101% under optimal conditions. The use of SOM as an exploratory analysis tool was an effective approach for selection of the most appropriate internal standards.

Automated summary

This study investigated the behaviors of internal standards with different flow rates of the cell collision gas (He) for the determination of Cd, Pb, Pd, Pt, Rh, and Sn in fish and mollusk samples by ICP-MS. Addition and recovery experiments were conducted to evaluate the method's accuracy, with self-organizing map (SOM) proving to be an effective tool for selecting the most appropriate internal standards.