Journal
TALANTA
Volume 225, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2020.121903
Keywords
Ion mobility spectrometry; Carbohydrate; Isomer separation; Metal cation; Halogen anion; Noncovalent adduct
Categories
Funding
- National Natural Science Foundation of China [61971248]
- National Key Research and Development Program of China [2017YFC1001700]
- Key Research and Development Program of Zhejiang Province [2020C03064]
- Science and Technology Major Project of Ningbo [2018B10075]
- Special Research Funding from the Marine Biotechnology and Marine Engineering Discipline Group in Ningbo University [422004582]
- K.C. Wong Magna Fund in Ningbo University
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The study systematically investigated the effect of alkaline earth cations and halogen anions on the separation of isomeric carbohydrates, finding that the [M + Ca + I]+ complex was the optimum complex for enhancing IMS separation resolution. This complex showed effective separation capability for all 21 pairs of isomeric oligosaccharides used in the study and could potentially be used as an effective complex for enhancing IMS separation resolution of isomeric carbohydrates.
Comprehensive analysis of carbohydrates, one of the critical steps towards the fundamental understanding of biological systems, has been hindered by the identifications of their complex isomeric structures differing in monosaccharide constituents, linkage positions, and configurations. While ion mobility spectrometry-mass spectrometry (IMS-MS) based methods have shown their utilities in performing glycomics measurements, there is still a lack of analytical methods allowing effective isomeric carbohydrate separation. Here, we systematically investigated the effect of alkaline earth cations (Ca2+ and Ba2+) and halogen anions (Cl, Br and I) addition on the separation of 21 pairs of isomeric oligosaccharides using trapped ion mobility spectrometry-mass spectrometry (TIMS-MS). [M + Ca + I](+) complex (M is an oligosaccharide) has shown experimentally to be the optimum complex for the separation of all 21 pairs of isomeric oligosaccharides used in this study with a calculated average resolution (r) similar to 1.82 and resolving power (R) similar to 100-150. The separation capability of this complex was further demonstrated by using mixtures of 15 oligosaccharides. The optimum [M + Ca + I](+) complex can be potentially used as an effective complex for enhancing the IMS separation resolution of isomeric carbohydrates.
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