Generation of two gene edited iPSC-lines carrying a DOX-inducible NGN2 expression cassette with and without GFP in the AAVS1 locus

Neurog2 is the gene encoding the neuronal transcription factor NGN2, which can convert stem cells into functional neurons in a fast and efficient way. Here we report the generation of two iPS cell lines, where DOX inducible constructs of neurog2 either without or with T2A-eGFP were inserted into the safe-site locus AAVS1. These iPS cell lines, BIONi010-C-13 and BIONi010-C-15, respectively, stay pluripotent without DOX but differentiate to (GFP positive) neurons when DOX is added without the need of differentiation factors.

Automated summary

Neurog2 is a gene encoding the neuronal transcription factor NGN2, converting stem cells into functional neurons efficiently. Two iPS cell lines, BIONi010-C-13 and BIONi010-C-15, were generated with DOX-inducible constructs of neurog2, allowing differentiation into neurons when DOX is added.