Journal
STEM CELL RESEARCH
Volume 53, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scr.2021.102339
Keywords
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Funding
- Natural Science Foundation of China [81801278]
- China Scholarship Council [201608130015]
- Natural Science Foundation of Hebei Province [H2019206637]
- Key Natural Science Foundation of Hebei Province [H2020206557]
- Hebei University Science and technology research project [ZD2019049]
- Overseas researcher Program in Hebei Provincial Department of human resources and social security [C20190509]
- Hebei Medical University
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A human induced pluripotent stem cell (iPSC) line was successfully derived from skin cells of a 48-year old male donor, which was integration-free, had a normal karyotype, expressed pluripotency markers, and showed potential for trilineage differentiation in vitro. This iPSC model will be valuable for studying physiological processes, drug validation, and serving as a control in pathological mechanistic studies.
In this study, skin biopsy was collected from a healthy 48-year old male donor with informed consent, and the fibroblasts were isolated from the dermal explant cultures. Here, a human induced pluripotent stem cell (iPSC) line was derived from the fibroblasts using the reprogramming four Yamanaka factors (Oct3/4, Sox2, Klf4, c-Myc). The generated iPSCs were integration-free, displayed the normal karyotype, expressed pluripotency markers and demonstrated trilineage differentiation potential in vitro. This iPSC model will be useful for investigating physiological processes, drug validation as well as a control in pathological mechanistic studies.
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