Journal
STEM CELL RESEARCH
Volume 53, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scr.2021.102267
Keywords
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Funding
- National Key R&D Program of China [2017YFA0105000]
- National Natural Science Foundation of China [U1904207, 81530037, 91849115, 82001973]
- Provincial and Ministry of Health Construction Committee of Henan Province [SBGJ2020003017]
- Excellent Youth Foundation of Henan Health Commission
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Through successful iPSC construction and validation from a female with an APP gene mutation, this study provides new prospects for Alzheimer's disease treatment and research.
Alzheimer's disease (AD) is a neurodegenerative disease that manifests mainly as cognitive, behavioral, and neuropsychiatric changes and impairs social functions and activities of daily living. The beta-amyloid precursor protein (APP) gene is one of the most common pathogenic genes associated with AD. We isolated dermal fibroblasts from a 51-year-old woman with an APP gene mutation (c.1756G > A). The induced pluripotent stem cells (iPSCs) were successfully constructed by transferring the reprogramming plasmids expressing OCT3/4, SOX2, KLF4, LIN28, and L-MYC. The generated iPSC line was pluripotent, as verified by immunofluorescence, flow cytometry, and teratoma formation test. The iPSC line will have broad prospects in drug screening, cell transplantation, and gene therapy.
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