One-step synthesis of red emission multifunctional carbon dots for label-free detection of berberine and curcumin and cell imaging

In this work, the red emission multifunctional carbon dots (R-CDs) were prepared via one-pot hydrothermal strategy of neutral red (NR) and ethylenediamine (EDA) for the label-free detection of berberine and curcumin, cell imaging, and fluorescent flexible film. The as-fabricated R-CDs not only possess good water dispersibility and excellent fluorescence stability, but also were successfully employed as a photoluminescent nanoprobe for label-free monitoring of berberine (BRH) and curcumin (Cur) based on dynamic quenching and internal filter effect (IFE), respectively. More importantly, as-proposed R-CDs displayed outstanding cellular permeability and lower cytotoxicity for cellular applications, which was consistent with the results of confocal fluorescence imaging and cell viability measurement of SMMC7721 cells. Thus, the multifunctional R-CDs may provide a rich tool library for biosensing and cellular imaging reagent applications. Interestingly, R-CDs were also used to manufacture R-CDs/PVA composites as fluorescent flexible films. To the best of our knowledge, this is the first demonstration of a label-free multifunctional fluorescent nanoprobe for berberine and curcumin based on red emission CDs. CO 2021 Elsevier B.V. All rights reserved.

Automated summary

In this study, red emission multifunctional carbon dots were successfully prepared through a simple hydrothermal method for label-free detection of berberine and curcumin, cell imaging, and manufacturing fluorescent flexible films. The multifunctional carbon dots exhibited good water dispersibility, stable fluorescence performance, excellent cellular permeability, and low cytotoxicity, making them promising for various applications in biosensing and cellular imaging.