Application of ANOVA-simultaneous component analysis to quantify and characterise effects of age, temperature, syrup adulteration and irradiation on near-infrared (NIR) spectral data of honey

NIR spectroscopy combined with chemometric analysis has proven to be a rapid and cost-effective screening tool for the detection of syrup-adulterated honey. Processing and storage conditions which alter the chemical and physical state of honey may affect the spectra. The effects of age, storage temperature, syrup adulteration (10 and 20% w/w) and irradiation treatment on the NIR spectra of honey were investigated as a function of time with ANOVA-simultaneous component analysis (ASCA), an experimental design-focused exploratory data analysis method. The factors ?time?, ?temperature? and ?adulteration? were found to have significant effects (p < 0.05), but no significant effect was observed for irradiation treatment. A significant interaction effect was found between factors ?time? and ?adulteration?, with the greatest disparity between authentic and adulterated class signals found immediately after adulteration and decreasing within three months thereafter. NIR spectroscopy combined with chemometric analysis has proven to be a rapid and cost-effective screening tool for the detection of syrup-adulterated honey. Processing and storage conditions which alter the chemical and physical state of honey may affect the spectra. The effects of age, storage temperature, syrup adulteration (10 and 20% w/w) and irradiation treatment on the NIR spectra of honey were investigated as a function of time with ANOVA-simultaneous component analysis (ASCA), an experimental design-focused exploratory data analysis method. The factors 'time', 'temperature' and 'adulteration' were found to have significant effects (p < 0.05), but no significant effect was observed for irradiation treatment. A significant interaction effect was found between factors 'time' and 'adulteration', with the greatest disparity between authentic and adulterated class signals found immediately after adulteration and decreasing within three months thereafter. (c) 2021 Elsevier B.V. All rights reserved.

Automated summary

NIR spectroscopy combined with chemometric analysis is an effective tool for detecting syrup-adulterated honey. Factors such as age, storage temperature, syrup adulteration, and irradiation treatment can significantly affect the NIR spectra of honey, with time and adulteration having the greatest impact on signal disparity.