4.7 Article

Electrochemical detection of telomerase in cancer cells based on the in-situ formation of streptavidin-biotin-DNA-biotin networks for signal amplification

Journal

SENSORS AND ACTUATORS B-CHEMICAL
Volume 334, Issue -, Pages -

Publisher

ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2021.129659

Keywords

Telomerase; Electrochemical biosensor; Streptavidin; Biotin; HeLa cells

Funding

  1. Program for Innovative Research Team of Science and Technology in the University of Henan Province [21IRTSTHN005]
  2. National Natural Science Foundation of China [21905002]

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This study presents a simple and sensitive electrochemical strategy for detecting telomerase extracted from HeLa cells, utilizing the signal amplification of in-situ formed streptavidin-biotin-DNA-biotin networks. The method effectively amplifies the signal by creating an insulating layer on a gold electrode surface, thus enabling the detection of telomerase.
This work reports on a simple and sensitive electrochemical strategy for the detection of telomerase extracted from HeLa cells. It was based on the signal amplification of in-situ performed streptavidin-biotin-DNA-biotin (SA-biotin-DNA-biotin) networks. Specifically, the DNA primer immobilized on a gold electrode was elongated by telomerase. After the hybridization of biotin-DNA-biotin to the extended primer, the in-situ formation of SA-biotin-DNA-biotin networks was initiated on the electrode surface within 10 min through the SA-biotin interactions. The resulting networks created an insulating layer, thus hampering the electron transfer and causing the significant increase in the electrochemical impedance. Telomerase extracted from 2 HeLa cells can be readily measured. The method showed a linear relationship in the range of 50 similar to 5000 cells/mL. The signal-amplified strategy can be applied to develop novel sensing platforms for the detection of different biomarkers.

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