Journal
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
Volume 84, Issue -, Pages 116-122Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejps.2016.01.019
Keywords
Antitumoral; Chalcone; Glioma; Polyphenol; Apoptosis; NF-kappa B
Categories
Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [484181/2012-2]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS)
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Treated glioblastoma multiforme (GBM) patients only survive 6 to 14 months after diagnosis; therefore, the development of novel therapeutic strategies to treat gliomas remains critically necessary. Considering that phenolic compounds, like quercetin, have the potential to be used in the chemotreatment of gliomas and that some flavonoids exhibit the ability to cross the BBB, in the present study, we investigated the antitumor effect of flavonoids (including chalcones, flavones, flavanones and flavonols). Initially their activities were tested in C6 glioma cells screened using the MTT method, resulting in the selection of chalcone 2 whose feasibility was confirmed by a Trypan Blue exclusion assay in the low mu M range on C6 glioma cells. Cell cycle and apoptotic death analyses on C6 glioma cells were also performed, and chalcone 2 increased the apoptosis of the cells but did not alter the cell cycle progression. In addition, treatments with these two compounds were not cytotoxic to hippocampal organotypic cultures, amodel of healthy neural cells. Furthermore, the results indicated that 2 induced apoptosis by inhibition of NF-kappa B and activation of active caspase-3 in glioma cells, suggesting that it is a potential prototype to develop new treatments for GBM in the future. (C) 2016 Elsevier B.V. All rights reserved.
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