Journal
SCIENTIA HORTICULTURAE
Volume 280, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.scienta.2021.109899
Keywords
Tissue culture; Scion; Rootstock; Graft union; Micropropagation
Categories
Funding
- Callaghan Innovation R&D Fellowship Grant
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In-vitro grafting involves grafting a micro-scion onto a micro-rootstock under aseptic conditions, offering advantages over traditional grafting methods. It has been primarily used for producing virus-free plants and studying scion-rootstock interactions, long-distance signaling, and virus-indexing.
In-vitro grafting involves grafting a micro-scion onto a micro-rootstock under aseptic conditions. Due to juvenility and health status of the source plants for microscion / micromotstock, in-vitro grafting possesses many advantages over traditional field / bench grafting methods. So far, in-vitro grafting has been used for producing virusfree plants as well as studying scion-rootstock interactions, long-distance signalling and virus-indexing. However, this technique has other potential applications, such as propagation of fruit trees and crop improvement, which has been neglected. This article reviews recent applied methodologies and their findings in the last 20 years, and discusses challenges and future direction of in-vitro grafting.
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