Increased expression of antibiotic-resistance genes in biofilm communities upon exposure to cetyltrimethylammonium bromide (CTAB) and other stress conditions

Quaternary ammonium compounds (QAC, e.g., cetyltrimethylammonium bromide, (CTAB)) are widely used as surfactants and disinfectants. QAC already are commonly found in wastewaters, and their concentration could increase, since QAC are recommended to inactivate the SARS-CoV-2 (COVID-19) virus. Exposure of bacteria to QAC can lead to proliferation of antibiotic resistance genes (ARG). In particular, O-2-based membrane biofilm reactors (O-2-MBfRs) achieved excellent CTAB biodegradation, but ARG increased in their biofilms. Here, we applied meta-transcriptomic analyses to assess the impacts of CTAB exposure and operating conditions on microbial community's composition and ARG expression in the O-2-MBfRs. Two opportunistic pathogens, Pseudomonas aeruginosa and Stenotrophomonas maltophilia, dominated the microbial communities and were associated with the presence of ARG. Operating conditions that imposed stress on the biofilms, i.e., limited supplies of O-2 and nitrogen or a high loading of CTAB, led to large increases in ARG expression, particularly for genes conferring antibiotic-target protection. Important within the efflux pumps was the Resistance-Nodulation-Division (RND) family, which may have been active in exporting CTAB from cells. Oxidative stress appeared to be the key factor that triggered ARG proliferation by selecting intrinsically resistant species and accentuating the expression of ARG. Our findings suggest that means to mitigate the spread of ARG, such as shown here in a O-2-based membrane biofilm reactor, need to consider the impacts of stressors, including QAC exposure and stressful operating conditions. (C) 2020 Elsevier B.V. All rights reserved.

Automated summary

Quaternary ammonium compounds (QAC) are commonly used as surfactants and disinfectants, and may lead to proliferation of antibiotic resistance genes (ARG) in bacteria. Stressful operating conditions, such as limited oxygen and nitrogen supply or high concentrations of QAC, can significantly increase ARG expression, particularly for genes conferring antibiotic-target protection. The study highlights the importance of considering the impacts of stressors, including QAC exposure and operating conditions, in mitigating the spread of ARG in microbial communities.