Quorum sensing shaped microbial consortia and enhanced hydrogen recovery from waste activated sludge electro-fermentation on basis of free nitrous acid treatment

In this study, the feasibility of free nitrous acid (FNA) pretreatment coupled with quorum sensing (QS) was investigated to enhance hydrogen recovery from waste activated sludge (WAS) via electro-fermentation (EF). 3-oxo-hexanoyl-homoserine lactone (30C6-HSL), as the signal molecule, was only added in the first three cycles of sludge inoculation at the phase of microbial electrolysis cells (MECs) startup. Results showed that QS combined FNA (AHL-FMEC) enabled highest hydrogen yield and current (4.3 mg/g VSS and 4.5 mA), while that generated from sole FNA/QS treated WAS (FMEC/AHL-RMEC) were only 3.5/3.0 mg/g VSS and 1.5/1.5 mA, respectively. Fourier transform infrared (FT-IR) spectra illustrated the effective conversion of organics in AHL-FMEC, the utilization efficiencies of proteins and carbohydrates achieved to 75.0% and 79.7%, respectively. Besides, the internal resistance decreased from 34.5 Omega (FMEC) to 22.9 Omega (AHL-RMEC), further to 18.0 Omega, indicating the promoted bioelectrochemical activity of electroactive bacteria (EAB) in AHL-FMEC. Correspondingly, both EAB (21.7%), e.g., Geobacter (9.3%) and Pseudomonas (3.2%) and anaerobic fermentation bacteria (AFB, 28.6%), e.g., Proteiniclasticum (14.2%) and Petrimonas (3.6%) enriched to peaks in AHL-FMEC. Moreover, molecular ecological network (MEN) analysis revealed the underling relationships among AFB, EAB and homoacetogen in EF system, suggesting the possible cooperative QS has been constructed. The results obtained in this study may provide a new insight for efficient hydrogen recovery from electro-fermentation of WAS. (C) 2020 Elsevier B.V. All rights reserved.

Automated summary

The study explored the feasibility of utilizing free nitrous acid pretreatment combined with quorum sensing to enhance hydrogen recovery from waste activated sludge via electro-fermentation. The results showed that the combination of quorum sensing and free nitrous acid enabled the highest hydrogen yield and current, indicating a potential cooperative relationship among anaerobic fermentation bacteria, electroactive bacteria, and homoacetogens in the electro-fermentation system.