Journal
SCIENCE
Volume 372, Issue 6542, Pages 642-+Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.abf7945
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Funding
- EXSCALATE4CoV EU-H2020 Emergency Project [101003551]
- Cluster of Excellence Advanced Imaging of Matter of the Deutsche Forschungsgemeinschaft (DFG) [EXC 2056, 390715994]
- Helmholtz Association [ExNet-0002, InternLabs-0011]
- Federal Ministry of Education and Research (BMBF) [05K16GUA, 05K19GU4, 05K20BI1, 05K20FL1, 16GW0277, 031B0405D]
- Joachim-Herz-Stiftung Hamburg (project Infecto-Physics)
- Data Science in Hamburg, HELMHOLTZ, Graduate School for the Structure of Matter [HIDSS-0002 DASHH]
- DFG [INST 187/621-1, INST 187/686-1]
- Slovenian Research Agency [P1-0048, IO-0048]
- Boehringer Ingelheim Foundation
- Free and Hanseatic City of Hamburg
- Federal Ministry of Health
- EU Horizon 2020 ERC StG-2017 [759661]
- BMBF RTK Struktur [01KI20391]
- BMBF Visavix [05K16BH1]
- Leibniz Association [SAW-2014-HPI-4]
- European Research Council (ERC) [759661] Funding Source: European Research Council (ERC)
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The study identified 37 compounds that bind to the SARS-CoV-2 main protease through a high-throughput x-ray crystallographic screen of two repurposing drug libraries. Among these compounds, one peptidomimetic and six nonpeptidic compounds showed antiviral activity in cell-based viral reduction assays at nontoxic concentrations. The identification of two allosteric binding sites presents potential targets for drug development against SARS-CoV-2.
The coronavirus disease (COVID-19) caused by SARS-CoV-2 is creating tremendous human suffering. To date, no effective drug is available to directly treat the disease. In a search for a drug against COVID-19, we have performed a high-throughput x-ray crystallographic screen of two repurposing drug libraries against the SARS-CoV-2 main protease (M-pro), which is essential for viral replication. In contrast to commonly applied x-ray fragment screening experiments with molecules of low complexity, our screen tested already-approved drugs and drugs in clinical trials. From the three-dimensional protein structures, we identified 37 compounds that bind to M-pro. In subsequent cell-based viral reduction assays, one peptidomimetic and six nonpeptidic compounds showed antiviral activity at nontoxic concentrations. We identified two allosteric binding sites representing attractive targets for drug development against SARS-CoV-2.
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