Bilateral visual projections exist in non-teleost bony fish and predate the emergence of tetrapods

In most vertebrates, camera-style eyes contain retinal ganglion cell neurons that project to visual centers on both sides of the brain. However, in fish, ganglion cells were thought to innervate only the contralateral side, suggesting that bilateral visual projections appeared in tetrapods. Here we show that bilateral visual projections exist in non-teleost fishes and that the appearance of ipsilateral projections does not correlate with terrestrial transition or predatory behavior. We also report that the developmental program that specifies visual system laterality differs between fishes and mammals, as the Zic2 transcription factor, which specifies ipsilateral retinal ganglion cells in tetrapods, appears to be absent from fish ganglion cells. However, overexpression of human ZIC2 induces ipsilateral visual projections in zebrafish. Therefore, the existence of bilateral visual projections likely preceded the emergence of binocular vision in tetrapods.

Automated summary

The study reveals that bilateral visual projections exist in non-teleost fishes, and that the developmental program specifying visual system laterality differs between fishes and mammals. The presence of ipsilateral projections in fish ganglion cells appears to be absent due to the lack of the Zic2 transcription factor.