HDAC6 Is Involved in the Histone Deacetylation of In Vitro Maturation Oocytes and the Reprogramming of Nuclear Transplantation in Pig

It remained unknown whether HDAC6 affected the histone deacetylation of in vitro maturation oocytes and the reprogramming of nuclear transplantation in pig. Our results indicated that HDAC6 specific inhibition did not affect overall HDAC activity and meiosis process, which increased histone H3K9/K14 and H4K8 acetylation of porcine in vitro maturation oocytes and pseudo-pronucleus embryos. HDAC6 inhibition also significantly enhanced the cleavage and blastocyst of nuclear transfer embryos (0.81 +/- 0.12 vs. 0.68 +/- 0.12 and 0.46 +/- 0.19; 0.73 +/- 0.13 vs. 0.63 +/- 0.18 and 0.40 +/- 0.16, P<0.05). The inhibition of HDAC6 significantly enhanced histone H3K9/K14 and H4K8 acetylation, and upregulated the OCT4 and CDX2 expressions (1.83 +/- 0.16 vs. 1.00 +/- 0.00 %; 2.07 +/- 0.09 vs. 1.00 +/- 0.00; P<0.05) in porcine SCNT blastocysts. Interestingly, HDAC6 inhibition significantly increased the pseudo-pronucleus volume during somatic cell reprogramming. Thus, HDAC6 was required for porcine histone deacetylation during the in vitro maturation and pseudo-pronucleus stages. HDAC6 inhibition improved the in vitro development of nuclear transfer embryos. HDAC6 may restrict the reprogramming of somatic nuclear transfer by regulating pseudo-pronucleus expansion. We need further research to confirm this in the future.

Automated summary

The inhibition of HDAC6 enhances the development of nuclear transfer embryos in pigs by increasing histone acetylation and key gene expression, without affecting overall HDAC activity and meiosis process. Furthermore, HDAC6 may play a role in restricting somatic nuclear transfer reprogramming by regulating pseudo-pronucleus expansion. Further research is needed to confirm these findings.