4.6 Article

Upregulated Talin1 synergistically boosts β-estradiol-induced proliferation and pro-angiogenesis of eutopic and ectopic endometrial stromal cells in adenomyosis

Journal

REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
Volume 19, Issue 1, Pages -

Publisher

BMC
DOI: 10.1186/s12958-021-00756-7

Keywords

Adenomyosis (ADS); beta-Estradiol (beta-E-2); Talin1; Adenomyotic eutopic and ectopic endometrial stromal cell (ADS_Eu_ESC and ADS_Ec_ESC); Proliferation; Pro-angiogenesis

Funding

  1. National Key Research and Development Program of China [2018YFC1004803]
  2. National Natural Science Foundation of China [81571412]

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The study revealed that the overexpression of Talin1 may cooperate with beta-E-2 to stimulate ADS endometrial stromal cell proliferation and neovascularization, synergistically promoting the growth and survival of ectopic lesions.
Adenomyosis (ADS) is an estrogen-dependent gynecological disease with unspecified etiopathogenesis. Local hyperestrogenism may serve a key role in contributing to the origin of ADS. Talin1 is mostly identified to be overexpressed and involved in the progression of numerous human carcinomas through mediating cell proliferation, adhesion and motility. Whether Talin1 exerts an oncogenic role in the pathogenesis of ADS and puts an extra impact on the efficacy of estrogen, no relevant data are available yet. Here we demonstrated that the adenomyotic eutopic and ectopic endometrial stromal cells (ADS_Eu_ESC and ADS_Ec_ESC) treated with beta-estradiol (beta-E-2) presented stronger proliferative and pro-angiogenetic capacities, accompanied by increased expression of PCNA, Ki67, VEGFB and ANGPTL4 proteins. Meanwhile, these promoting effects were partially abrogated by Fulvestrant (ICI 182780, an estrogen-receptor antagonist). Aberrantly upregulation of Talin1 mRNA and protein level was observed in ADS endometrial specimens and stromal cells. Through performing functional experiments in vitro, we further determined that merely overexpression of Talin1 (OV-Talin1) also enhanced ADS stromal cell proliferation and pro-angiogenesis, while the most pronounced facilitating effects were found in the co-intervention group of OV-Talin1 plus beta-E-2 treatment. Results from the xenograft nude mice model showed that the hypodermic endometrial lesions from co-intervention group had the highest mean weight and volume, compared with that of individual OV-Talin1 or beta-E-2 treatment. The expression levels of PCNA, Ki67, VEGFB and ANGPTL4 in the lesions were correspondingly elevated the most in the co-intervention group. Our findings unveiled that overexpressed Talin1 might cooperate with beta-E-2 in stimulating ADS endometrial stromal cell proliferation and neovascularization, synergistically promoting the growth and survival of ectopic lesions. These results may be beneficial to provide a new insight for clarifying the pathogenesis of ADS.

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