Journal
PROCESS BIOCHEMISTRY
Volume 103, Issue -, Pages 78-86Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2021.02.007
Keywords
Bacillus subtilis; Denitrification; Genome sequencing; Functional gene
Categories
Funding
- National Key Research and Development Program of China [2018YFA0900300]
- Natural Science Foundation of Jiangsu Province [BE2018055]
- Fishery Science and Technology Projects in Jiangsu Province [Y2018-26]
- Wuxi Science and Technology Project [CLE02N1713]
- China Postdoctoral Science Foundation [2020M671330]
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The aerobic denitrifying Bacillus subtilis JD-014 showed efficient nitrogen removal capability in a lab-scale continuous experiment, with maximum removal efficiencies of NO3--N and NO2--N reaching 98.91% and 81.99% respectively. Analysis of the complete genome revealed 43 putative genes associated with nitrogen pathways, of which 9 selective putative genes were validated to participate in the denitrification process.
Bacillus is a species that has been suggested to contain abundant denitrifying strains. The aerobic denitrifying Bacillus subtilis JD-014 isolated previously was found to exhibit efficient nitrogen removal capability with outstanding tolerance to nitrite toxicity. However, the bioremediation potential in real treatment process has not been verified. Meanwhile, although several functional denitrifying genes have been screened, the comprehensive mechanism of denitrification pathway for JD-014 remains unclear. Herein, a lab-scale continuous experiment was conducted to investigate the application potential of JD-014, which demonstrated that JD-014 could effectively deplete nitrogen pollution, with the maximum removal efficiency of NO3--N and NO2--N of 98.91 % and 81.99 %, respectively. Furthermore, the complete genome analysis of JD-014 revealed the denitrification pathways in genetic levels. 43 putative genes are associated with the nitrogen pathways, among which 9 selective putative genes were validated to participate in the denitrification process. The present work suggests that JD-014 has great potential of practical application in bioremediation of nitrogen wastewater. The understanding of nitrogen metabolic pathways also provides significant molecular information for further Bacillus species modification and development.
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