4.8 Article

Nanobody cocktails potently neutralize SARS-CoV-2 D614G N501Y variant and protect mice

Related references

Note: Only part of the references are listed.
Article Multidisciplinary Sciences

Spike mutation D614G alters SARS-CoV-2 fitness

Jessica A. Plante et al.

Summary: The D614G substitution in the SARS-CoV-2 spike protein enhances viral replication and infectivity in human lung epithelial cells, primary airway tissues, and hamsters. This variant may increase transmission in the upper respiratory tract and doesn't seem to significantly reduce vaccine efficacy. Further research on therapeutic antibodies targeting the circulating G614 virus is recommended.

NATURE (2021)

Article Biochemistry & Molecular Biology

UCSF ChimeraX: Structure visualization for researchers, educators, and developers

Eric F. Pettersen et al.

Summary: UCSF ChimeraX is a powerful visualization program with enhanced performance and graphics, offering new tools and analysis features, support for various areas like virtual reality, and improved ease of use, along with an app store for researchers to contribute new tools.

PROTEIN SCIENCE (2021)

Article Biochemistry & Molecular Biology

Nanobody generation and structural characterization of Plasmodium falciparum 6-cysteine protein Pf12p

Melanie H. Dietrich et al.

Summary: Surface-associated proteins play essential roles in the life cycle of Plasmodium parasites, with the 6-cysteine (6-cys) protein family being a major target for vaccine development. Recent research has provided insights into the crystal structure of the 6-cys protein Pf12p and the generation of specific nanobodies, offering new tools for studying the diverse functions of the 6-cys protein family in the Plasmodium life cycle.

BIOCHEMICAL JOURNAL (2021)

Article Microbiology

Complete Mapping of Mutations to the SARS-CoV-2 Spike Receptor-Binding Domain that Escape Antibody Recognition

Allison J. Greaney et al.

Summary: Antibodies targeting the SARS-CoV-2 spike receptor-binding domain (RBD) are key in neutralizing antibody responses, and a deep mutational scanning method was used to assess the impact of all amino-acid mutations in the RBD on antibody binding with 10 human monoclonal antibodies. The study identified the clustered escape mutations in different surfaces of the RBD that correspond to structurally defined antibody epitopes, showing that even antibodies targeting the same surface can have distinct escape mutations.

CELL HOST & MICROBE (2021)

Article Multidisciplinary Sciences

Structure-guided multivalent nanobodies block SARS-CoV-2 infection and suppress mutational escape

Paul-Albert Koenig et al.

Summary: In this study, new neutralizing nanobodies targeting the SARS-CoV-2 spike protein were developed, showing enhanced neutralizing activity through engineering technology and successfully suppressing the emergence of escape mutants. The findings suggest that nanobodies can neutralize through receptor binding competition and also render virions noninfectious.

SCIENCE (2021)

Article Biochemistry & Molecular Biology

The antigenic anatomy of SARS-CoV-2 receptor binding domain

Wanwisa Dejnirattisai et al.

Summary: Antibodies play a crucial role in immune protection against SARS-CoV-2, with some being used as therapeutics. A study identified 377 human monoclonal antibodies, focusing on 80 that bind the virus spike, and found that most highly inhibitory antibodies can block the virus-receptor interaction. Novel binding modes of potent inhibitory antibodies were discovered, showing potential for prophylactic or therapeutic use in animal models.
Article Biochemistry & Molecular Biology

In Silico Investigation of the New UK (B.1.1.7) and South African (501Y.V2) SARS-CoV-2 Variants with a Focus at the ACE2-Spike RBD Interface

Bruno O. Villoutreix et al.

Summary: The SARS-CoV-2 virus exploits ACE2 as a receptor for cell invasion, with certain mutations potentially enhancing its transmission capabilities and pathogenicity through effects on ACE2 binding. The UK strain may exhibit higher affinity for ACE2, while the transmission level of the South African strain could be influenced by the N501Y substitution.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Article Biology

The development of Nanosota-1 as anti-SARS-CoV-2 nanobody drug candidates

Gang Ye et al.

Summary: Nanosota-1, a series of single-domain antibodies, has shown to effectively combat SARS-CoV-2 virus by binding to the receptor-binding domain, blocking viral receptor ACE2, and demonstrating preventive and therapeutic efficacy against live infection. Produced at high yields in bacteria and with exceptional thermostability, Nanosota-1 may potentially contribute to the battle against COVID-19 as an effective and inexpensive drug candidate.

ELIFE (2021)

Article Multidisciplinary Sciences

A pneumonia outbreak associated with a new coronavirus of probable bat origin

Peng Zhou et al.

NATURE (2020)

Article Multidisciplinary Sciences

Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2

Renhong Yan et al.

SCIENCE (2020)

Article Multidisciplinary Sciences

Human neutralizing antibodies elicited by SARS-CoV-2 infection

Bin Ju et al.

NATURE (2020)

Article Multidisciplinary Sciences

Cross-neutralization ofSARS-CoV-2 by a human monoclonal SARS-CoV antibody

Dora Pinto et al.

NATURE (2020)

Article Multidisciplinary Sciences

Structural basis of receptor recognition by SARS-CoV-2

Jian Shang et al.

NATURE (2020)

Article Biochemistry & Molecular Biology

Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant

Leonid Yurkovetskiy et al.

Article Multidisciplinary Sciences

Convergent antibody responses to SARS-CoV-2 in convalescent individuals

Davide F. Robbiani et al.

NATURE (2020)

Article Multidisciplinary Sciences

Potent neutralizing antibodies against multiple epitopes on SARS-CoV-2 spike

Lihong Liu et al.

NATURE (2020)

Article Multidisciplinary Sciences

Potently neutralizing and protective human antibodies against SARS-CoV-2

Seth J. Zost et al.

NATURE (2020)

Article Biochemistry & Molecular Biology

Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2

Jiangdong Huo et al.

NATURE STRUCTURAL & MOLECULAR BIOLOGY (2020)

Article Multidisciplinary Sciences

Adaptation of SARS-CoV-2 in BALB/c mice for testing vaccine efficacy

Hongjing Gu et al.

SCIENCE (2020)

Article Multidisciplinary Sciences

Structure-based design of prefusion-stabilized SARS-CoV-2 spikes

Ching-Lin Hsieh et al.

SCIENCE (2020)

Article Multidisciplinary Sciences

Studies in humanized mice and convalescent humans yield a SARS-CoV-2 antibody cocktail

Johanna Hansen et al.

SCIENCE (2020)

Article Multidisciplinary Sciences

Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability

Philip J. M. Brouwer et al.

SCIENCE (2020)

Article Multidisciplinary Sciences

Structural basis of a shared antibody response to SARS-CoV-2

Meng Yuan et al.

SCIENCE (2020)

Article Multidisciplinary Sciences

SARS-CoV-2 neutralizing antibody structures inform therapeutic strategies

Christopher O. Barnes et al.

NATURE (2020)

Article Multidisciplinary Sciences

Structures and distributions of SARS-CoV-2 spike proteins on intact virions

Zunlong Ke et al.

NATURE (2020)

Article Multidisciplinary Sciences

Ultrapotent human antibodies protect against SARS-CoV-2 challenge via multiple mechanisms

M. Alejandra Tortorici et al.

SCIENCE (2020)

Article Multidisciplinary Sciences

An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction

Leo Hanke et al.

NATURE COMMUNICATIONS (2020)

Article Multidisciplinary Sciences

Humanized single domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding domain

Xiaojing Chi et al.

NATURE COMMUNICATIONS (2020)

Article Multidisciplinary Sciences

Development of humanized tri-specific nanobodies with potent neutralization for SARS-CoV-2

Jianbo Dong et al.

SCIENTIFIC REPORTS (2020)

Article Multidisciplinary Sciences

SARS-CoV-2 D614G variant exhibits efficient replication ex vivo and transmission in vivo

Yixuan J. Hou et al.

SCIENCE (2020)

Article Multidisciplinary Sciences

An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike

Michael Schoof et al.

SCIENCE (2020)

Article Multidisciplinary Sciences

Versatile and multivalent nanobodies efficiently neutralize SARS-CoV-2

Yufei Xiang et al.

SCIENCE (2020)

Article Multidisciplinary Sciences

Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2

Tania F. Custodio et al.

NATURE COMMUNICATIONS (2020)

Review Immunology

Measuring immunity to SARS-CoV-2 infection: comparing assays and animal models

David S. Khoury et al.

NATURE REVIEWS IMMUNOLOGY (2020)

Article Biochemistry & Molecular Biology

Unexpected Receptor Functional Mimicry Elucidates Activation of Coronavirus Fusion

Alexandra C. Walls et al.

Article Medicine, Research & Experimental

Delivery of ALX-0171 by inhalation greatly reduces respiratory syncytial virus disease in newborn lambs

Alejandro Larios Mora et al.

Letter Biochemical Research Methods

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

Shawn Q. Zheng et al.

NATURE METHODS (2017)

Article Biochemical Research Methods

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

Ali Punjani et al.

NATURE METHODS (2017)

Review Pharmacology & Pharmacy

Nanobodies®† as inhaled biotherapeutics for lung diseases

Gino Van Heeke et al.

PHARMACOLOGY & THERAPEUTICS (2017)

Article Biochemistry & Molecular Biology

Gctf: Real-time CTF determination and correction

Kai Zhang

JOURNAL OF STRUCTURAL BIOLOGY (2016)

Review Virology

Structure, Function, and Evolution of Coronavirus Spike Proteins

Fang Li

ANNUAL REVIEW OF VIROLOGY, VOL 3 (2016)

Article Biochemistry & Molecular Biology

Stock-based detection of protein oligomeric states in jsPISA

Eugene Krissinel

NUCLEIC ACIDS RESEARCH (2015)

Article Biochemical Research Methods

A general protocol for the generation of Nanobodies for structural biology

Els Pardon et al.

NATURE PROTOCOLS (2014)

Review Biochemistry & Molecular Biology

Nanobodies: Natural Single-Domain Antibodies

Serge Muyldermans

ANNUAL REVIEW OF BIOCHEMISTRY, VOL 82 (2013)

Article Biochemical Research Methods

MolProbity: all-atom structure validation for macromolecular crystallography

Vincent B. Chen et al.

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY (2010)

Article Biochemical Research Methods

XDS

Wolfgang Kabsch

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY (2010)

Article Biochemical Research Methods

Features and development of Coot

P. Emsley et al.

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY (2010)

Article Biochemical Research Methods

PHENIX: a comprehensive Python-based system for macromolecular structure solution

Paul D. Adams et al.

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY (2010)

Article Biochemistry & Molecular Biology

IMGT/V-QUEST: the highly customized and integrated system for IG and TR standardized V-J and V-D-J sequence analysis

Xavier Brochet et al.

NUCLEIC ACIDS RESEARCH (2008)

Article Biochemical Research Methods

PHENIX:: building new software for automated crystallographic structure determination

PD Adams et al.

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY (2002)