Parallel evolution of UbiA superfamily proteins into aromatic O-prenyltransferases in plants

Plants produce ?300 aromatic compounds enzymatically linked to prenyl side chains via C?O bonds. These O-prenylated aromatic compounds have been found in taxonomically distant plant taxa, with some of them being beneficial or detrimental to human health. Although their O-prenyl moieties often play crucial roles in the biological activities of these compounds, no plant gene encoding an aromatic O-prenyltransferase (O-PT) has been isolated to date. This study describes the isolation of an aromatic O-PT gene, CpPT1, belonging to the UbiA superfamily, from grapefruit (Citrus ? paradisi, Rutaceae). This gene was shown responsible for the biosynthesis of O-prenylated coumarin derivatives that alter drug pharmacokinetics in the human body. Another coumarin O-PT gene encoding a protein of the same family was identified in Angelica keiskei, an apiaceous medicinal plant containing pharmaceutically active O-prenylated coumarins. Phylogenetic analysis of these O-PTs suggested that aromatic O-prenylation activity evolved independently from the same ancestral gene in these distant plant taxa. These findings shed light on understanding the evolution of plant secondary (specialized) metabolites

Automated summary

Plants produce around 300 aromatic compounds enzymatically linked to prenyl side chains via C-O bonds, some of which can impact human health. The discovery of aromatic O-PT genes in plants like grapefruit and Angelica keiskei sheds light on the evolution of plant secondary metabolites. Phylogenetic analysis suggests that aromatic O-prenylation activity evolved independently from the same ancestral gene in distant plant taxa.