Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 118, Issue 21, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2014242118
Keywords
GTPase-activating protein; BCH domain; Rho; Sec14; signaling
Categories
Funding
- Ministry of Education (MOE) Singapore [R154-000-625-112]
- National University of Singapore Academic Research Fund (AcRF) Tier 1 [R154000683112, R154000C07114, R154-000-A72-115]
- Mechanobiology Institute Singapore
- Singapore Ministry of Education AcRF Tier 3 Grant [MOE2016T31002]
- National Institute of General Medical Sciences from the NIH [P30 GM124165]
- DOE Office of Science [DEAC0206CH11357]
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The spatiotemporal regulation of signaling cascades is essential for various biological pathways and is controlled by scaffolding proteins. The BCH domain targets small GTPases and their regulators, playing a crucial role in cell elongation, retraction, and membrane protrusion during cell migration, myoblast differentiation, and neuritogenesis. Analysis of the BCH domain of p50RhoGAP revealed a dimeric structure with unique RhoA-binding loop and lipid-binding pocket, providing insights into the regulation of Rho activity. Additionally, a destabilizing mutation in the BCH domain triggers the release of the adjacent GAP domain, leading to RhoA inactivation and increased self-association of p50RhoGAP molecules.
Spatiotemporal regulation of signaling cascades is crucial for various biological pathways, under the control of a range of scaffolding proteins. The BNIP-2 and Cdc42GAP Homology (BCH) domain is a highly conserved module that targets small GTPases and their regulators. Proteins bearing BCH domains are key for driving cell elongation, retraction, membrane protrusion, and other aspects of active morphogenesis during cell migration, myoblast differentiation, and neuritogenesis. We previously showed that the BCH domain of p50RhoGAP (ARHGAP1) sequesters RhoA from inactivation by its adjacent GAP domain; however, the underlying molecular mechanism for RhoA inactivation by p50RhoGAP remains unknown. Here, we report the crystal structure of the BCH domain of p50RhoGAP Schizosaccharomyces pombe and model the human p50RhoGAP BCH domain to understand its regulatory function using in vitro and cell line studies. We show that the BCH domain adopts an intertwined dimeric structure with asymmetric monomers and harbors a unique RhoA-binding loop and a lipid-binding pocket that anchors prenylated RhoA. Interestingly, the 05-strand of the BCH domain is involved in an intermolecular 0-sheet, which is crucial for inhibition of the adjacent GAP domain. A destabilizing mutation in the 05-strand triggers the release of the GAP domain from autoinhibition. This renders p50RhoGAP active, thereby leading to RhoA inactivation and increased self-association of p50RhoGAP molecules via their BCH domains. Our results offer key insight into the concerted spatiotemporal regulation of Rho activity by BCH domain-containing proteins.
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