Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 118, Issue 12, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2023613118
Keywords
crossover; interference; heterochiasmy; synaptonemal complex; meiosis
Categories
Funding
- Max Planck Society
- Institut Jean-Pierre Bourgin's (IJPB) Plant Observatory technological platforms
- Saclay Plant Sciences [ANR-17-EUR-0007]
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The spatial organization of transverse filaments is essential for CO formation during meiosis, with the absence of ZYP1 leading to an unexpected increase in CO formation. The tripartite synaptonemal complex plays a key role in regulating the number and distribution of COs, affecting CO interference and heterochiasmy.
Meiotic crossovers (COs) have intriguing patterning properties, including CO interference, the tendency of COs to be well-spaced along chromosomes, and heterochiasmy, the marked difference in male and female CO rates. During meiosis, transverse filaments transiently associate the axes of homologous chromosomes, a process called synapsis that is essential for CO formation in many eukaryotes. Here, we describe the spatial organization of the transverse filaments in Arabidopsis (ZYP1) and show it to be evolutionary conserved. We show that in the absence of ZYP1 (zyp1a zyp1b null mutants), chromosomes associate in pairs but do not synapse. Unexpectedly, in absence of ZYP1, CO formation is not prevented but increased. Furthermore, genome-wide analysis of recombination revealed that CO interference is abolished, with the frequent observation of close COs. In addition, heterochiasmy was erased, with identical CO rates in males and females. This shows that the tripartite synaptonemal complex is dispensable for CO formation and has a key role in regulating their number and distribution, imposing CO interference and heterochiasmy.
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